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Transcriptional analysis of tyrosinase gene expression during bufo bufo development
Joint Authors
Cesare, Patrizia
Bonfigli, Antonella
Poma, Anna Maria
Colafarina, Sabrina
Zarivi, Osvaldo
Miranda, Michele
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 1 (31 Jan. 2019), pp.8-19, 12 p.
Publisher
Publication Date
2019-01-31
Country of Publication
Saudi Arabia
No. of Pages
12
Main Subjects
Topics
Abstract EN
Tyrosinase (EC.1.14.18.1.) is a widespread enzyme, in the phylogenetic scale, that produces melanin, from bacteria to man, by using as substrates monophenols, o-diphenols and molecular oxygen.
In this work we have confirmed and demonstrated that during Bufo bufo development tyrosinase activity and gene expression first occur at developmental stages 17–18 (tail bud-muscular response) as detected by a spectrophotometric assay and qRT-PCR.
As expected, also during B.
bufo development tyrosinase gene is expressed after the late gastrula (stage 12), differently from Rana pipiens development when tyrosinase mRNA appears at the neural plate stage and enzyme activity at stage 20 (gill circulation).
We have cloned and sequenced the B.
bufo tyrosinase cDNA in order to prepare B.
bufo tyrosinase cDNA specific primers (forward and reverse).
Tyrosinase mRNA cloning has been performed by using degenerate primers prepared according to the anuran tyrosinase gene sequence coding for the copper binding sites.
The expressions of tyrosinase gene and enzymatic activity during B.
bufo development support that until the developmental stage 17, embryo melanin is of maternal origin and at this stage can start embryo melanin synthesis.
A correlation exists between tyrosinase expression and O2 consumption during B.
bufo development
American Psychological Association (APA)
Cesare, Patrizia& Bonfigli, Antonella& Miranda, Michele& Poma, Anna Maria& Colafarina, Sabrina& Zarivi, Osvaldo. 2019. Transcriptional analysis of tyrosinase gene expression during bufo bufo development. Saudi Journal of Biological Sciences،Vol. 26, no. 1, pp.8-19.
https://search.emarefa.net/detail/BIM-896605
Modern Language Association (MLA)
Cesare, Patrizia…[et al.]. Transcriptional analysis of tyrosinase gene expression during bufo bufo development. Saudi Journal of Biological Sciences Vol. 26, no. 1 (Jan. 2019), pp.8-19.
https://search.emarefa.net/detail/BIM-896605
American Medical Association (AMA)
Cesare, Patrizia& Bonfigli, Antonella& Miranda, Michele& Poma, Anna Maria& Colafarina, Sabrina& Zarivi, Osvaldo. Transcriptional analysis of tyrosinase gene expression during bufo bufo development. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 1, pp.8-19.
https://search.emarefa.net/detail/BIM-896605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 18-19
Record ID
BIM-896605