Cytogenetic and genetic study of a Y-linked microsatellite polymorphism in polish black-and-white cattle breed

Abstract EN

The aim of the current study was to characterize Polish Black-and-White cattle by morphological study of the Y chromosome.

A total of 14 Y-linked microsatellites from UMN and INRA group were genotyped and assessed for polymorphism in a total 22 bulls.

Cytogenetic studies in Polish Black-and-White bulls showed the existence of two morphological forms of Y chromosome.

Among the 22 karyotypic analyzed bulls, 12 had submetacentric and 10 metacentric Y chromosome.

The centromeric index of Y chromosome measured as percentage length of the p arm to total length ratio in the first case was 28 ± 3.97% and in the second 47 ± 7.28%, whereas the relative size of these chromosomes remained within the same range.

Morphology and G- and C-banding patterns of both forms of Y chromosome were typical for other cattle breeds originating from Bos taurus.

Out of a total of 14 microsatellite loci examined, 13 showed specific alleles for two forms of Y chromosome.

In a pool of 62 alleles, 43 (69.3%) were common in the two groups of cattle, 19 (30.7%) can be considered as specific for the group; among them 8 were typical for metacentric group of Y chromosome and 11 for submetacentric.