The molecular detection of hpv infection in samples of Iraqi women with abnormal cervical smears
Other Title(s)
الكشف الجزيئي عن الإصابة بفيروس الورم الحليمي البشري في مسحات غير طبيعية لعنق الرحم لعينات مأخوذة من نساء عراقيات
Joint Authors
Abd al-Samad, Mays Numayr
Qandala, Nuha Juzif
Source
Issue
Vol. 59, Issue 4B (31 Dec. 2018), pp.1995-2004, 10 p.
Publisher
University of Baghdad College of Science
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
Human papillomavirus (HPV) types 16 and 18 cause almost 70% of cervical cancer cases worldwide.
Recently, testing for high-risk HPV types have been adopted by clinical practices for the early detection of cervical cancer in conjunction with cytology tests.
Cervical swab samples were collected at the Outpatient Gynecology department of Baghdad Teaching Hospital.
These samples consisted of a patient group of 50 samples, and a healthy control group of 10 samples.
A papanicolaou test (abbreviated as a Pap test) was also performed for each woman to examine the epithelial cells of both the endocervix and the upper vaginal region.
Total DNA (genomic, mitochondrial, and viral) was extracted from cervical swab samples for molecular studies.
HPV DNA testing was first done by using Real-Time PCR technology to target the L1 region of HR-HPV with specifically designed primers.
This was followed by using AmpliSens kit for specific detection and genotyping of HPV16 and 18 with multiplex Real-Time PCR.
The results of RT-PCR detection revealed that out of 16 samples detected with high-risk HPV, 5 samples were shown to be infected with HPV-16 and 5 samples were shown to be infected with HPV-18.
These results show a significant relationship between the histological outcome of the patient and persistent HPV infection.
American Psychological Association (APA)
Abd al-Samad, Mays Numayr& Qandala, Nuha Juzif. 2018. The molecular detection of hpv infection in samples of Iraqi women with abnormal cervical smears. Iraqi Journal of Science،Vol. 59, no. 4B, pp.1995-2004.
https://search.emarefa.net/detail/BIM-896924
Modern Language Association (MLA)
Abd al-Samad, Mays Numayr& Qandala, Nuha Juzif. The molecular detection of hpv infection in samples of Iraqi women with abnormal cervical smears. Iraqi Journal of Science Vol. 59, no. 4B (2018), pp.1995-2004.
https://search.emarefa.net/detail/BIM-896924
American Medical Association (AMA)
Abd al-Samad, Mays Numayr& Qandala, Nuha Juzif. The molecular detection of hpv infection in samples of Iraqi women with abnormal cervical smears. Iraqi Journal of Science. 2018. Vol. 59, no. 4B, pp.1995-2004.
https://search.emarefa.net/detail/BIM-896924
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2003-2004
Record ID
BIM-896924