Inhibition of tumor growth and angiogenesis by 2-(4-aminophenyl) benzothiazole in orthotopicglioma C6 rat model
Joint Authors
Lei, De Qiang
Deng, Xing Li
Zhao, Hong Yang
Zhang, Fang Cheng
Liu, Ru En
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 7 (30 Nov. 2018), pp.1483-1487, 5 p.
Publisher
Publication Date
2018-11-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
In the present study antitumor effect of 2-(4-aminophenyl) benzothiazole (BTZ) was evaluated against human U251 and rat C6 glioma cell lines using MTT assay.
It was observed that BTZ exhibited significant antitumor effect with IC50 of 3.5 and 4 mM against human U251 and rat C6 glioma cells respectively.
To gain in-depth insights about the antitumor effect of BTZ, glioma xenograft rat model was prepared.
The rats were treated with 10 mg and 15 mg/kg body weight doses of BTZ daily for 21 days after C6 cell administration.
Treatment of the rats with BTZ reduced the tumor volume to 12% compared to 100% in the untreated rats.
TUNEL assay showed a remarkable increase in the proportion of apoptotic cells in the BTZ treated rats than those in the untreated rats.
The increase in the population of apoptotic cells was 23-fold compared to control.
Immuno-histological staining revealed marked reduction (16%) in the proportion of CD31-stained vessels in the BTZ treated rats than those of the untreated rats.
These changes were accompanied with decreased transcript levels of vascular endothelial growth factor (VEGF) and the VEGF receptor Flt1 as well as ERK1/2 and matrix metalloproteinase-2 (MMP2).
Moreover, BTZ altered the expression of several cell cycle control proteins.
While as pRb protein expression decreased, E2F1 remained unaltered and cyclin D1 protein and p53 expression was enhanced.
Taken together, the results indicate that BTZ is a potent inhibitor of glioma cell proliferation in vivo and exerts its effects on cell cycle control and angiogenesis related proteins
American Psychological Association (APA)
Lei, De Qiang& Deng, Xing Li& Zhao, Hong Yang& Zhang, Fang Cheng& Liu, Ru En. 2018. Inhibition of tumor growth and angiogenesis by 2-(4-aminophenyl) benzothiazole in orthotopicglioma C6 rat model. Saudi Journal of Biological Sciences،Vol. 25, no. 7, pp.1483-1487.
https://search.emarefa.net/detail/BIM-897163
Modern Language Association (MLA)
Lei, De Qiang…[et al.]. Inhibition of tumor growth and angiogenesis by 2-(4-aminophenyl) benzothiazole in orthotopicglioma C6 rat model. Saudi Journal of Biological Sciences Vol. 25, no. 7 (Nov. 2018), pp.1483-1487.
https://search.emarefa.net/detail/BIM-897163
American Medical Association (AMA)
Lei, De Qiang& Deng, Xing Li& Zhao, Hong Yang& Zhang, Fang Cheng& Liu, Ru En. Inhibition of tumor growth and angiogenesis by 2-(4-aminophenyl) benzothiazole in orthotopicglioma C6 rat model. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 7, pp.1483-1487.
https://search.emarefa.net/detail/BIM-897163
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1486-1487
Record ID
BIM-897163