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Quantitative detection of Camv-35s promoter and T-Nos terminator in genetic modified tomato from Iraqi markets
Other Title(s)
للطماطم المحورة وراثيا في الأسواق العراقية Nos-T و الفاصل 35S-CaMV الكشف الكمي عن المشغل
Joint Authors
Sad al-Din, Shuruq Muhammad Kazim
Abbas, Muhammad Riyad
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 49, Issue 5 (31 Oct. 2018), pp.913-920, 8 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2018-10-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Aim of this research was quantitative detection of Increase and decrease in copy number of CaMV-35S promoter and Nos terminator in genetic modified tomato by using Real-time PCR.
Twenty four samples of genetic modified tomato seeds were isolated from 84 tomato samples collected from Iraqi markets during the period from December 2016 to January 2017.
The experiences were conducted in the Institute of Genetic Engineering, University of Baghdad.
Go Taq®qPCR master mix kit supplied by USA Promega Company, three specific primers to CaMV-35S promoter, T-Nos terminator and β-actin housekeeping gene supplied by Canadian Alpha Company were used.
To quantitative detection of increase and decrease in copy number of GM tomato samples contain CaMV-35S promoter and T-Nos terminator, comparing with the β-actin (houskeeping gene) using Multiple of Median (MoM) equation.
The results showed that the lowest recorded of Ct value was (27.88) for CaMV-35S promoter gave an increase in copy number (1.1766) above the normal limit, while highest recorded of Ct value was (32.67) gave an increase in copy number (1.0350) above the normal limit.
The lowest recorded of Ct value was (27.35) for T-Nos terminator gave an increase in copy number (1.1600) above the normal limit, whereas highest recorded of Ct value was (32.82) gave a decrease in copy number (0.9920) under the normal limit
American Psychological Association (APA)
Abbas, Muhammad Riyad& Sad al-Din, Shuruq Muhammad Kazim. 2018. Quantitative detection of Camv-35s promoter and T-Nos terminator in genetic modified tomato from Iraqi markets. The Iraqi Journal of Agricultural Science،Vol. 49, no. 5, pp.913-920.
https://search.emarefa.net/detail/BIM-898172
Modern Language Association (MLA)
Abbas, Muhammad Riyad& Sad al-Din, Shuruq Muhammad Kazim. Quantitative detection of Camv-35s promoter and T-Nos terminator in genetic modified tomato from Iraqi markets. The Iraqi Journal of Agricultural Science Vol. 49, no. 5 (2018), pp.913-920.
https://search.emarefa.net/detail/BIM-898172
American Medical Association (AMA)
Abbas, Muhammad Riyad& Sad al-Din, Shuruq Muhammad Kazim. Quantitative detection of Camv-35s promoter and T-Nos terminator in genetic modified tomato from Iraqi markets. The Iraqi Journal of Agricultural Science. 2018. Vol. 49, no. 5, pp.913-920.
https://search.emarefa.net/detail/BIM-898172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 919-920
Record ID
BIM-898172