Molecular detection of Glucokinase (GCK) gene mutation in maturity-onset diabetes of the young (MODY2) among Sudanese families with type 2 diabetes mellitus
Joint Authors
Uthman, Abd al-Sadiq Abd al-Azim
al-Tayyib, Hisham Nur al-Dayim
Abd al-Hamid, Muzammil Muhammad
Yusuf, Jalal Muhammad
Source
Journal of Science and Technology : in Natural and Medical Sciences
Issue
Vol. 19, Issue 1 (30 Jun. 2018), pp.58-66, 9 p.
Publisher
Sudan University of Science and Technology Deanship of Scientific Research
Publication Date
2018-06-30
Country of Publication
Sudan
No. of Pages
9
Main Subjects
Topics
Abstract EN
Maturityonset diabetes of the young (MODY) is an autosomal dominant form of non-insulin dependent diabetes mellitus (NIDDM), it‟s a heterogeneous group of disorders characterized by dysfunction of beta-cells, and usually referred to monogenic forms of diabetes mellitus to distinguish them from the common types of disease such as type 1 or type 2 diabetes.
(M.
Vaxillaire et al.
2008 .Aim The aim of this study was to examine the prevalence and nature of mutations in GCK gene among Sudanese families.
Exons 7were screened, which are specific for pancreatic glucokinase, mutations at positions 682A>G, p.T228A; in 160 subjects (80 patients suspected to have MODY2 and 80 healthy controls without family history of diabetes mellitus).
By using polymerase chain reaction (PCR) the target part of GCK gene was amplified, the amplified regions were digested by using restriction enzymes: BstUI and electrophoresed on agarose gel.
Results of mutation were screened using restriction fragment length polymorphism and confirm by using DNA sequencing.
The results showed that, there wasno any association of these mutations in Sudanese suspected MODY2 patients or in healthy controls.
On the other hand during analysis of the DNA sequences observed three synonymous mutations, in one family that may have significant effect on the protein function In conclusion, the study indicates that mutations in GCK/MODY2 at position 682A>G, p.T228A is not detectedin Sudanese families
American Psychological Association (APA)
Uthman, Abd al-Sadiq Abd al-Azim& al-Tayyib, Hisham Nur al-Dayim& Abd al-Hamid, Muzammil Muhammad& Yusuf, Jalal Muhammad. 2018. Molecular detection of Glucokinase (GCK) gene mutation in maturity-onset diabetes of the young (MODY2) among Sudanese families with type 2 diabetes mellitus. Journal of Science and Technology : in Natural and Medical Sciences،Vol. 19, no. 1, pp.58-66.
https://search.emarefa.net/detail/BIM-898649
Modern Language Association (MLA)
Uthman, Abd al-Sadiq Abd al-Azim…[et al.]. Molecular detection of Glucokinase (GCK) gene mutation in maturity-onset diabetes of the young (MODY2) among Sudanese families with type 2 diabetes mellitus. Journal of Science and Technology : in Natural and Medical Sciences Vol. 19, no. 1 (2018), pp.58-66.
https://search.emarefa.net/detail/BIM-898649
American Medical Association (AMA)
Uthman, Abd al-Sadiq Abd al-Azim& al-Tayyib, Hisham Nur al-Dayim& Abd al-Hamid, Muzammil Muhammad& Yusuf, Jalal Muhammad. Molecular detection of Glucokinase (GCK) gene mutation in maturity-onset diabetes of the young (MODY2) among Sudanese families with type 2 diabetes mellitus. Journal of Science and Technology : in Natural and Medical Sciences. 2018. Vol. 19, no. 1, pp.58-66.
https://search.emarefa.net/detail/BIM-898649
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 64-66
Record ID
BIM-898649