Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene
Joint Authors
Ali, Zahrah Muhammad
Banun, Shayma R.
Source
al-Kufa University Journal for Biology
Issue
Vol. 10, Issue 2 (31 Aug. 2018), pp.157-167, 11 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2018-08-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
One hundred and eight of soil samples have been collected from 17 area loci in Maysan governorate during the period from September to the October 2012 ,and cultivated on lauria agar media for isolation and identification of B.
licheniformis.
Primary identification was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then the isolates was identified by VITEK-2 compact system Version 05.01 using BCL identification cards (BCL-ID).
The results revealed that 53(94.64%) of isolates identified as Bacillus licheniformis which could be classified in classes where the very good identification class with percent of identification reached to 43.39%, followed by good , acceptable, and low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as unidentified organism.
The isolates were identified using the PCR technique for detection of gyrase B gene clarify that 56(100%) isolates of B.
licheniformis with gyrase B gene that encodes the subunit B protein of DNA gyrase
American Psychological Association (APA)
Banun, Shayma R.& Ali, Zahrah Muhammad. 2018. Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene. al-Kufa University Journal for Biology،Vol. 10, no. 2, pp.157-167.
https://search.emarefa.net/detail/BIM-899002
Modern Language Association (MLA)
Banun, Shayma R.& Ali, Zahrah Muhammad. Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene. al-Kufa University Journal for Biology Vol. 10, no. 2 (2018), pp.157-167.
https://search.emarefa.net/detail/BIM-899002
American Medical Association (AMA)
Banun, Shayma R.& Ali, Zahrah Muhammad. Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene. al-Kufa University Journal for Biology. 2018. Vol. 10, no. 2, pp.157-167.
https://search.emarefa.net/detail/BIM-899002
Data Type
Journal Articles
Language
English
Record ID
BIM-899002