Green synthesis of spherical gold nanoparticles by chitosan for 6 mercaptopurine delivery
Joint Authors
Shuman, Samiyah A.
Badr, Y. A.
Husayn, Amna
al-Salam, Mahmud A.
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 51, Issue 4 (31 Oct. 2018), pp.175-180, 6 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2018-10-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Topics
Abstract EN
In the present work, gold nanoparticles were successfully prepared using chitosan as reducing and capping agent and subsequently used as carriers for hydrophobic anticancer 6 mercaptopurine (6 MP).
The use of this drug is limited due to its poor bioavailability.
The purpose of the present investigation was to evaluate a method for loading hydrophobic drug 6 MP on chitosan reduced gold nanoparticles (AuNPs).
The resulting AuNPs and 6MP loaded AuNPs were characterized using ultraviolet visible spectrophotometry and transmission electron microscopy.
To estimate the improvement of the drug delivery of AuNPs, breast cancer cells MCF7 were treated with 6 MP and 6 MP loaded AuNPs and the cell viability was studied in vitro using Sulphorhodamine-B (SRB) assay.
The data showed that both free 6 MP and 6 MP loaded AuNPs revealed anti-proliferation activity against the cancer cells in dose-dependent manners.
Moreover, 6 MP loaded AuNPs displays significantly greater anti-proliferation activity than that of free 6 MP at all concentrations and IC50 1.93 nM.
The improvement of antiproliferation activity of 6 MP loaded AuNPs compared to that of 6 MP can be attributed to enhanced intracellular uptake of the functionalized GNPs via the mechanism of endocytosis.
American Psychological Association (APA)
Husayn, Amna& Shuman, Samiyah A.& Badr, Y. A.& al-Salam, Mahmud A.. 2018. Green synthesis of spherical gold nanoparticles by chitosan for 6 mercaptopurine delivery. Arab Journal of Nuclear Sciences and Applications،Vol. 51, no. 4, pp.175-180.
https://search.emarefa.net/detail/BIM-899039
Modern Language Association (MLA)
Husayn, Amna…[et al.]. Green synthesis of spherical gold nanoparticles by chitosan for 6 mercaptopurine delivery. Arab Journal of Nuclear Sciences and Applications Vol. 51, no. 4 (Oct. 2018), pp.175-180.
https://search.emarefa.net/detail/BIM-899039
American Medical Association (AMA)
Husayn, Amna& Shuman, Samiyah A.& Badr, Y. A.& al-Salam, Mahmud A.. Green synthesis of spherical gold nanoparticles by chitosan for 6 mercaptopurine delivery. Arab Journal of Nuclear Sciences and Applications. 2018. Vol. 51, no. 4, pp.175-180.
https://search.emarefa.net/detail/BIM-899039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 179-180
Record ID
BIM-899039