The association of human herpes virus simplex 1and 2 infection of male genital tract and male infertility

Abstract EN

Objectives: To investigate the association of viral infections present in the lower genital tract of males and relationship among male infertility.

Methods: ELISA and RT-PCR technique of 100 semen and serum specimens, collected over 8 months from males investigated for infertility, were retrospectively assessed, by screening anti-human Herpes Simplex Virus (HSV) IgM and IgG in the serum and detection of HSV DNA in seminal fluid.

Results: One hundred seminal fluid and blood samples were taken from men and investigated for infertility over a period of 8 months from July 2016 to February 2017.

The seminal fluids and serum of patients mentioned to the laboratory from the fertility clinics of Kamal AL-samarai hospital in Baghdad and outer clinics.

The investigations were conducted to find the HSV detection rate in the seminal fluid of infertile men and to evaluate the impact of the viral infection on the major sperm parameters.

Blood and seminal fluid were collected from 100 infertile male.

The results were shown that 20/100 (20.0%) were positive for IgM and 77/100 (77.0%) were positive for IgG of HSV-1; for HSV-2, positive IgM and IgG antibodies were 9/100 (9.0%) and 54/100 (54.0%), respectively.

The PCR showed that out of 100 samples 13 (13.0%) gave positive results for HSV-1 while 12 (12.0%) for HSV-2, In case of control group, results were (3.0%),(4.0%) positive for anti-HSV-1, HSV-2 IgM antibodies respectively, and (27.0%), (10.0%) positive for anti-HSV-1, HSV-2 IgG antibodies respectively in control group, using ELISA