Frequently isolated bacteria from lactating cows with clinical and subclinical mastitis in Gharbia governorate, Egypt

Abstract EN

This study was carried out on a total number of 122 mixed breed cows (46 clinical and 76 subclinical mastitic cows) aged between 2-8 years old and collected from 17 dairy farms and also single cases from farmers located in different localities in Gharbia, Egypt.

Bacteriological culturing revealed CNS isolated from clinical and subclinical cases 28 (60.9%), 19 (25%) respectively, CPS isolated from clinical cases only 9 (19.6%) and E.

coli isolated from clinical and subclinical cases 9 (19.6%), 30 (39.5%) respectively.

Serological identification of 10 E.

coli isolates revealed following serotypes (O26, O111, O114, O125, O146 and O166).

Sensitivity test for (CPS) showed that the highest resistance was to amoxicillin 66.7% and the lowest resistance was to Gentamycin and Sulphatrimethoprime 11%, (CNS) showed that the highest resistance was to Oxacillin 46.8% then Kanamycin 8.5% and E.

Coli showed that the highest resistance was to Oxacillin 59%, the lowest resistance was to Gentamycin 5.1%, complete susceptibility to Enrofloxacine, Ciprofloxacine and high susceptibility to Gentamycin and Sulphatrimethoprime.

Multiplex PCR of (CPS) isolates resulted in all isolates give a positive reaction at 279bp of nuc genes (Staphylococcus aureus) (S.

aureus) meanwhile 2 isolates 1.6% (2/122) give a positive reaction at 147bp of mecA genes (methicillin resistance S.

aureus) (MRSA).

The objective of this study is to isolate and identify the common bacterial isolates from mixed breed mastitic cows, detection the antibiotics sensitivity of the studied bacteria and molecular detection of nuc genes (S.

aureus specific primers) and mecA genes (methicillin resistance S.

aureus specific primers) in (CPS).