Clinical diagnosis and molecular characterizations of sheep pasteurellosis

Abstract EN

Pasteurella multocida and Mannheimia haemolytica are the known bacterial pathogens, which cause severe respiratory diseases of sheep.

So, this work intended to detect Pasteurella pathogens involved in sheep respiratory infections using culturing method, biochemical tests and PCR assay.

To realize this, 75 samples of nasal swabs collected from clinically diseased sheep at Sohag Governorate.

These samples subjected to bacteriological examination and the result showed that, 17 ( 22.7%) samples of the 75examined samples were positive to pasteurella spp.( P.

multocida positive samples were15 ( 88.2%) and P.haemolytica positive samples were 2 ( 11.7%) on blood agar and biochemical tests.

PCR using KMT1 gene (P.

multocida) and ssa gene (P.

haemolytica) specific primers, was carried out on these 17 positive samples.

The results of PCR proved that only 3 (17.6%) isolates were positive for P.

multocid and 2 (11.8%) isolates were positive for P.

heamolytica.

Considering of this fact this study suggest screening and detecting Pasteurella.Spp.

By using of traditional methods and PCR technique in pneumonic sheep.