Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood

Abstract EN

The activity of urease purified from Proteus mirabilis bacteria was estimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained as standard in size (20- 40)nm, purity 99%.

The enzyme activity was estimated by incubating the pure enzyme with various concentrations of nanoparticles ranged between (1- 6) μg/ml.

The results indicates a decrease in enzymatic activity with increasing of nanoparticles concentration.

After that, the concentration of blood urea was measured using urease obtained from a standard kit, urease purified from Proteus mirabilis and the urease- gamma iron nanoparticles solution.

The comparison was then made among the results of urea concentrations by statistical analysis using T-test.

The results showed that there is no significant difference between the results obtained from urease standard kit and urease purified from the bacteria, this is due to the efficiency of urease purified from the bacteria.

On the other hand, the results showed that there is a significant difference (P≤ 0.01) in urea concentrations obtained from urease- gamma iron oxide nanoparticles solution due to the inhibition of the enzyme which lost its activity by nanoparticles