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Detection a new antiseptic resistant variant of qac gene in some multi drug resistant staphylococcus aureus isolated from different clinical sources
Other Title(s)
التحري عن المورث الجديد المقاوم للمطهر بين عزلات المكورات العنقودية الذهبية المتعددة المقاومة للمضادات الحيوية و المعزولة من مصادرسريرية مختلفة
Joint Authors
Sabir, Nur
Muhammad, Hibah Abd al-Amir
Qandala, Nuha Juzif
Source
Issue
Vol. 16, Issue 3 (30 Sep. 2019), pp.571-579, 9 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2019-09-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
The increasing use of antiseptic compounds creates selective pressure cause emergence of antiseptic resistance among Staphylococcus aureus .Resistance mechanism of antiseptic is driven mainly by multi drug resistant (MDR) efflux protein.Sixty five isolates of S.aureuswere collected from different clinical sources and subjected to 11 antibiotics most of them are recognized by efflux systems as extruded substrates.
Range of efflux activity was estimated using cartwheel method.
Simultaneous discrimination of antiseptic coding genes (qacA/B, smr and norA)as well as nuc and mecA genes among multidrug resistantS.aureus(MRSA) isolates was preformed using multiplex PCR assay , 61 isolatesamong 65 were positive tonucand mecA genes, 58 of them were positive to norA, 14 of them were positive to qacA/B and only two were positive to smr.
All isolates detected with qacA/B characterized by fluoroquinolones resistant and most of them show strong efflux activity at cartwheel assay, all of the 14 isolates positive qacA/B were sequenced to differentiate between variants depending on position 323 (aspartic in QacA, alanine in QacB), 3 of them harbored asparagines amino acid at position 323 and considered to be a new variants that reported for the first time.
American Psychological Association (APA)
Sabir, Nur& Qandala, Nuha Juzif& Muhammad, Hibah Abd al-Amir. 2019. Detection a new antiseptic resistant variant of qac gene in some multi drug resistant staphylococcus aureus isolated from different clinical sources. Baghdad Science Journal،Vol. 16, no. 3, pp.571-579.
https://search.emarefa.net/detail/BIM-899587
Modern Language Association (MLA)
Sabir, Nur…[et al.]. Detection a new antiseptic resistant variant of qac gene in some multi drug resistant staphylococcus aureus isolated from different clinical sources. Baghdad Science Journal Vol. 16, no. 3 (2019), pp.571-579.
https://search.emarefa.net/detail/BIM-899587
American Medical Association (AMA)
Sabir, Nur& Qandala, Nuha Juzif& Muhammad, Hibah Abd al-Amir. Detection a new antiseptic resistant variant of qac gene in some multi drug resistant staphylococcus aureus isolated from different clinical sources. Baghdad Science Journal. 2019. Vol. 16, no. 3, pp.571-579.
https://search.emarefa.net/detail/BIM-899587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 577-578
Record ID
BIM-899587