Kinetic studies of Na+-K+-ATPase in tissue aerobic thyroid patients
Other Title(s)
دراسة حركية لأنزيم صوديوم- بوتاسيوم أتبيز K+-ATPase-Na المنقى من نسيج مرضى الدرقية الهوائية
Joint Authors
al-Samirrai, Suzan Jamil Ali
al-Shayi, Rana Rad Zanzal Muhammad
Source
Issue
Vol. 16, Issue 3 (sup) (30 Sep. 2019), pp.740-746, 7 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2019-09-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
Na+/K+-ATPase is a prevalent enzyme that maintains the Na+ and K+ gradients across the cell membrane by transporting three Na+ out and two K+ into the cell, the aim of this study is to provide detailed mechanistic insights, potentially with important effects on physiological regulation of active Na and K transport in tissues of Aerobic Thyroid Patient.
Thyroid tissues were obtained from a 35 year old patients, the operation was carried out at the Al-Hadi Specialist Hospital in Samarra city, the sample was stored at -20ºC until used.
The purification protocol included Salt Precipitation, Ion Exchange Chromatography, Gel Filtration and Electrophoresis, a spectrophotometric method was used to determine the enzyme activity.
kinetic parameters was also obtained for the enzyme.
Partial purification of Na+/K+-ATPase revealed two isoenzymes (I ,II).
The purity of separated isoenzymes were proved by SDS-PAGE electrophoresis.
The kinetic characteristics of Na+/K+-ATPase showed that optimum substrate concentration about 1.5mM, Km 1.052mM, and Vmax 6.062, optimum temperature was 37 ºC, optimum pH 7.4 and optimum time in 25 min.
Na+/K+-ATPase purified from Thyroid tissue has distinct kinetic characteristic that reflects the importance of intracellular regulation of specific Na+/K+-ATPase pump which gives cells the ability to precisely coordinate to their physiological requirements .
American Psychological Association (APA)
al-Samirrai, Suzan Jamil Ali& al-Shayi, Rana Rad Zanzal Muhammad. 2019. Kinetic studies of Na+-K+-ATPase in tissue aerobic thyroid patients. Baghdad Science Journal،Vol. 16, no. 3 (sup), pp.740-746.
https://search.emarefa.net/detail/BIM-899919
Modern Language Association (MLA)
al-Samirrai, Suzan Jamil Ali& al-Shayi, Rana Rad Zanzal Muhammad. Kinetic studies of Na+-K+-ATPase in tissue aerobic thyroid patients. Baghdad Science Journal Vol. 16, no. 3 (Supplement) (2019), pp.740-746.
https://search.emarefa.net/detail/BIM-899919
American Medical Association (AMA)
al-Samirrai, Suzan Jamil Ali& al-Shayi, Rana Rad Zanzal Muhammad. Kinetic studies of Na+-K+-ATPase in tissue aerobic thyroid patients. Baghdad Science Journal. 2019. Vol. 16, no. 3 (sup), pp.740-746.
https://search.emarefa.net/detail/BIM-899919
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 744-745
Record ID
BIM-899919