![](/images/graphics-bg.png)
Characterization and immobilization of purified polyphenol oxidase extracted from banana peel
Joint Authors
Aziz, Ghazi M.
al-Sadi, Ali J.
Bidan, Dalal S.
Source
Iraqi Journal of Biotechnology
Issue
Vol. 17, Issue 2 (30 Sep. 2018), pp.13-22, 10 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2018-09-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
This study was undertaken to obtain a crude extract of polyphenol oxidase (PPO) from banana peel in addition to purification and characterization to determining the optimal temperature, pH, the storage periods , number of used times and dyes decolrization.
A partial purified polyphenol oxidase from banana peel was immobilized by agarose and Calcium Alginates.
The entrapment of PPO by the Calcium alginate showed the best method for the immobilization.
The immobilized and free PPO enzyme properties were studied.
The results of study showed the optimal pH of free and immobilized PPO enzyme activities from banana peel were 7.0; the optimal pH of free PPO enzyme stability ranged from 6.0 - 7.0.
The optimal pH of immobilized PPO enzyme stability ranged between7 - 8.
The optimal temperatures of free and immobilized PPO activity extracted from banana peel were 35ᴼC and the free enzyme was stabilized in temperature 35 ᵒC then the enzyme activity began to decrease and it was lost at 60 ᵒC; the immobilized enzyme remained stable in temperature ranged 35-55 ᵒC.
The immobilized PPO activity which incubated for more times with catechol decreased after ninth time of using, the experiment showed that the activity of immobilized enzyme decreased with long storage period.
The neutral red, acridine and toluidine were decolorized and showed a change in their absorbance values after the immobilized PPO incubate for a time while no analysis occurred for other dyes.
American Psychological Association (APA)
Aziz, Ghazi M.& al-Sadi, Ali J.& Bidan, Dalal S.. 2018. Characterization and immobilization of purified polyphenol oxidase extracted from banana peel. Iraqi Journal of Biotechnology،Vol. 17, no. 2, pp.13-22.
https://search.emarefa.net/detail/BIM-900289
Modern Language Association (MLA)
Aziz, Ghazi M.…[et al.]. Characterization and immobilization of purified polyphenol oxidase extracted from banana peel. Iraqi Journal of Biotechnology Vol. 17, no. 2 (Sep. 2018), pp.13-22.
https://search.emarefa.net/detail/BIM-900289
American Medical Association (AMA)
Aziz, Ghazi M.& al-Sadi, Ali J.& Bidan, Dalal S.. Characterization and immobilization of purified polyphenol oxidase extracted from banana peel. Iraqi Journal of Biotechnology. 2018. Vol. 17, no. 2, pp.13-22.
https://search.emarefa.net/detail/BIM-900289
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 21-22
Record ID
BIM-900289