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Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue
Other Title(s)
تقييم طرق عزل لخلايا أولية من نسيج الجلد للكارب العادي (Cyprinus carpio)
Author
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 32, Issue 2 (31 Dec. 2018), pp.119-125, 7 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
In this study, pieces of skin from common carp (Cyprinus carpio L.) fish were collected freshly from juveniles from a privet fish farm in Duhok city, Kurdistan-Iraq.
Those pieces of skin were cultured In Vitro by using two methods of explanting; direct explants and enzymatic pre-digested explants of tissue fragments.
The results showed a highest cell yield (5×103 cell/ml) on passage 2 of enzymatic pre-digested tissues and higher cell growth rate in compare with cell yield (38×102 cell/ml) gained from direct explant tissues.
Both procedures showed forming of clumped cells colonies in initial passages (P1 and P2), and the morphological changes were developed in both cells to show a fibroblast like appearance thereafter.
Such findings are important and advantageous to suggest a faster and more efficient protocol for tissue culture cells isolation with lower cost and better isolation success as well.
American Psychological Association (APA)
Hassun, Mayada Fuad. 2018. Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 2, pp.119-125.
https://search.emarefa.net/detail/BIM-900347
Modern Language Association (MLA)
Hassun, Mayada Fuad. Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue. Iraqi Journal of Veterinary Sciences Vol. 32, no. 2 (2018), pp.119-125.
https://search.emarefa.net/detail/BIM-900347
American Medical Association (AMA)
Hassun, Mayada Fuad. Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 2, pp.119-125.
https://search.emarefa.net/detail/BIM-900347
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 125
Record ID
BIM-900347