![](/images/graphics-bg.png)
Isolation and detection of rotavirus by enzyme linked immune assay in fecal specimens of buffalo calves
Other Title(s)
عزل و تشخيص فايروس الروتا من عينات براز عجول الجاموس باستخدام اختبار الفحص المناعي المرتبط بالانزيم (الأليزا)
Joint Authors
Khalaf, Ahmad Sadi
al-Duri, Athir Abd al-Razzaq
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 42, Issue 2 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to June 2017.
All samples were examined by Immuno-chromatographic rapid tests for detection of buffalo rotavirus, twenty samples were positive.
Enzyme-Linked Immunosorbent Assay (ELISA) test was also used and revealed only 8 positive samples, the later samples were used for viral isolation on fetal bovine kidney cell culture for detection the cytopathogenic effects (CPE) of the virus.
The cytopathogenic effects of virus were induced rounded cells and gaps; as a result of rupture of these infected cells by rotavirus.
Moreover Enzyme-Linked Immunosorbent Assay (ELISA test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation.
The TCID50 was estimated for rotavirus on fetal bovine kidney cells 104.5 virus/0.05 ml
American Psychological Association (APA)
Khalaf, Ahmad Sadi& al-Duri, Athir Abd al-Razzaq. 2018. Isolation and detection of rotavirus by enzyme linked immune assay in fecal specimens of buffalo calves. Iraqi Journal of Veterinary Medicine،Vol. 42, no. 2, pp.1-6.
https://search.emarefa.net/detail/BIM-900377
Modern Language Association (MLA)
Khalaf, Ahmad Sadi& al-Duri, Athir Abd al-Razzaq. Isolation and detection of rotavirus by enzyme linked immune assay in fecal specimens of buffalo calves. Iraqi Journal of Veterinary Medicine Vol. 42, no. 2 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-900377
American Medical Association (AMA)
Khalaf, Ahmad Sadi& al-Duri, Athir Abd al-Razzaq. Isolation and detection of rotavirus by enzyme linked immune assay in fecal specimens of buffalo calves. Iraqi Journal of Veterinary Medicine. 2018. Vol. 42, no. 2, pp.1-6.
https://search.emarefa.net/detail/BIM-900377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 4-5
Record ID
BIM-900377