Effect of policosanol extract administration on lipid profile in hypercholestrolemic female rats during lactation

Abstract EN

The present study designed to investigate the effects of the extracted policosanol on lipid profile of hypercholestrolemic lactating female rats (Ratus norvigicus).

Forty eight female rat (10-12) weeks old,and weighting (200-250 gm) were used in the current study.

After daily vaginal smear for 3 sequences estrus cycles, they mated with twenty four healthy adult fertile males weighting (280-350 gm), 24 of these females were induced to be hypercholestrolemic during the pregnancy,and others still healthy.

Sugarcane were collected from Mesan province and policosanol was extracted from sugarcane wax.

Immediately after birth the animals were divided into 6 groups: Group 1 (n=8) (Control) : Normal rats were given only 0.5 ml/ animal of Dimethyl Sulphoxide (DMSO) daily immediately after birth.

Group 2 (n=8) hyperchol.

(HC): Hpercholestrolemic rats were given only DMSO 0.5 ml/ animal daily immediately after birth.

Group 3(n=8) (HC+SM): Hpercholestrolemic rats were given simvastatine at dose 20mg/kg BW/ day dissolved in DMSO orally by gavages' immediately after birth.

Group 4(n=8) (SM) : Normal rats were given only simvastatine at dose 20mg/kg BW/ day dissolvxsqqs ed in DMSO orally by gavages' immediately after birth.

Group 5 (n= 8) (HC+ST.

pol): Hpercholestrolemic rats were given standard policosanol at dose 20mg/kg BW/day orally by gavages' immediately after birth.

Finally group 6(n=8)( HC+EX.

pol): Hpercholestrolemic rats were given policosanol extraction at dose 20mg/kg BW/day orally by gavages immediately after birth.

The treatment continued until weaning ( 30 days).

All animals were then sacrificed and blood samples were collected for measuring of lipid parameters.

Total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglyceride (TG) in all groups .

Results revealed a significant improvement in TC level was recorded in (HC+SM) group and no difference was observed as compared with control group.

While level of TC in (SM), (HC+ST.pol) and (HC+EX.pol) treated groups decreased significantly (p ≤ 0.05) compared with control group, (HC) and (HC+SM) treated groups.

No significant differences in HDL-C was observed between (HC+EX.pol) and control group.

While the level of HDL-C still significantly lower in all other treated groups compared with control group.

A significant reduction (p ≤ 0.05) in LDL-C level was recorded in all treated groups compared with (HC) group.

No significant differences were observed in LDL-C between (SM), (HC+ST.

pol) and (HC+EX.pol) compared with control group.

While the LDL-C still significantly higher (p ≤ 0.05) in (HC+SM) compared with control group.

Levels of triglyceride (TG) in (HC+SM),(HC+ST.pol) and (HC+EX.pol) treated groups recorded a significant elevation ( p≤ 0.05) as compared with control group.

While levels of TG in (SM) treated group were significantly decreased (p≤0.05) as compared with control group.

Conclusion: local sugar cane can be used to extract the policosanol which has antihyperlipidemic effect on hypercholestrolemic lactating female rats