Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi
Other Title(s)
استخدام التقنيات الجزيئية كوسيلة بديلة لتشخيص و توصيف الثيليريا إكواي
Joint Authors
Surur, Shayma Subhi
al-Hawwari, Najwa Muhammad
Sulayman, Ahmad Mahmud
Hilmi, Nashwa Muhammad
al-Sayfi, Mahmud Abd al-Nabi Umar
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 32, Issue 1 (30 Jun. 2018), pp.5-11, 7 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
The purpose of this study was to determine the prevalence of clinical, subclinical and chronic infection with the equine parasite T.
eqiii in some Egyptian localities (Cairo and Giza governorates).
A panel of 396 equine blood samples representing 141 horses, 250 donkeys and 5 mules was collected from equines during the period from April 2015 to March 2016 using microscopic examination and conventional PCR.
Microscopically a twenty two (5.56%) of 396 were positive for T.
equi merozoites that appeared as small rounded, pyriform shaped and maltase cross shaped merozoites.
Among 8/141(5.67%) horses and 14/250 (5.60%) donkeys were found to have positive for T.equi.
A one hundred blood samples (45 horses, 50 donkeys and 5 mules) selected randomly were also examined by PCR.
The results of PCR showed 30/100(11/45 (24.4%) horses, 18/50 (36%) donkeys and 1/5 (20%) mule) were positive for T.equi.
When the sequenced PCR amplicons (n=3) were aligned to the reference nucleotide sequences of T.
equi accessed in Genbank, the horse isolate showed insertion of Thymine (T) base at position 23 and substitution of Thymine (T) base with Cytosine (C) base at position 91, while the donkey and mule isolates have no alterations when compared to the reference sequences.
The phylogenetic analysis showed that the sequenced PCR isolates belonged to T.equi.
The obtained sequences were deposited in the GeneBank database under accession numbers MF192854, MF192855 and MF192856.
American Psychological Association (APA)
al-Sayfi, Mahmud Abd al-Nabi Umar& Hilmi, Nashwa Muhammad& al-Hawwari, Najwa Muhammad& Surur, Shayma Subhi& Sulayman, Ahmad Mahmud. 2018. Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 1, pp.5-11.
https://search.emarefa.net/detail/BIM-900599
Modern Language Association (MLA)
al-Sayfi, Mahmud Abd al-Nabi Umar…[et al.]. Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi. Iraqi Journal of Veterinary Sciences Vol. 32, no. 1 (2018), pp.5-11.
https://search.emarefa.net/detail/BIM-900599
American Medical Association (AMA)
al-Sayfi, Mahmud Abd al-Nabi Umar& Hilmi, Nashwa Muhammad& al-Hawwari, Najwa Muhammad& Surur, Shayma Subhi& Sulayman, Ahmad Mahmud. Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 1, pp.5-11.
https://search.emarefa.net/detail/BIM-900599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10-11
Record ID
BIM-900599