Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection
Other Title(s)
تعدد أشكال جين هرمون النمو في نتائج التلقيح الصناعي لأبقار مادورا الملقحة بمني اليموزين كمرجع للاختيار الوراثي
Joint Authors
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 32, Issue 1 (30 Jun. 2018), pp.113-118, 6 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Research on genetic polymorphism of growth hormone (GH) and receptor growth hormone (rGH) has not been done in crossbred of Limousin cattle, so it is interesting to be examined.
Blood samples were taken from 14 Madura calves were artificially inseminated with Limousin cement.
DNA amplification is done by using Polymerase Chain Reaction (PC'R) method.
Restriction Fragment Length Polymorphism (RFLP) method to determine the genotype.
DNA sequencing was done to determine nucleotide sequences of GH unit genes.
The results showed that identification of GH and rGH gene polymorphisms was done by breaking DNA fragments from 432 and 298 bp in Maduia and Limousin cattle (Madrasin) ie.
L and V alleles have a frequency of 0.67 and 0.33 for the GH gene, respectively.
This proves that the crossed-breeding of Madrasin have V allele that is not owned by the Madura cattle.
While in the rGH gene, the A allele is 0.92 and the G allele is 0.08.
with the frequency of the A allele larger than the G allele.
This research concluded: that GH and rGH undergo changes on polymorphisms in Madrasin cattle can be used as a basis for selection.
American Psychological Association (APA)
Utomo, Budi& Safltri, Erima. 2018. Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 1, pp.113-118.
https://search.emarefa.net/detail/BIM-900689
Modern Language Association (MLA)
Utomo, Budi& Safltri, Erima. Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection. Iraqi Journal of Veterinary Sciences Vol. 32, no. 1 (2018), pp.113-118.
https://search.emarefa.net/detail/BIM-900689
American Medical Association (AMA)
Utomo, Budi& Safltri, Erima. Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 1, pp.113-118.
https://search.emarefa.net/detail/BIM-900689
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 118
Record ID
BIM-900689