Prevalence of streptococcus agalactiae colonization among pregnant women in Gaza City, Palestine

Other Title(s)

دراسة مدى انتشار البكتيريا العقدية القاطعة للدر بين النساء الحوامل في مدينة غزة، فلسطين

Dissertant

Isalim, Suhayr Ilayyan

Thesis advisor

al-Aylah, Nabil Abd Allah
al-Manamah, Abd al-Rauf Ali

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2017

English Abstract

Background: Streptococcus agalactiae is one of the most important causal agents of serious neonatal infections.

Early detection of perinatal rectovaginal carriage of Group B Streptococcus (GBS) is important in the prevention of newborn infections.

Objectives: To evaluate the prevalence of group B Streptococcal (GBS) colonization among pregnant women in Gaza city.

Also to determine the susceptibility pattern of GBS isolates against different antimicrobial agents.

In addition, to investigate possible risk factors for GBS colonization.

Methods: Two hundreds rectovaginal swabs collected from pregnant women from Al Shifa hospital were screened for GBS colonization.

Standard microbiological methods according to the Atlanta Centers for Disease Control and Prevention (CDC) recommendations were used to isolate and identify GBS from rectovaginal swabs.

Selective and chromogenic culture in addition only for 100 sample PCR was employed for the detection of GBS.

Antimicrobial susceptibility testing (AST) was performed according to CLSI guidelines on 42 GBS isolates using clindamycin, erythromycin, penicillin G, tetracycline and vancomycin.

Results: Out of 200 pregnant women, 42 (21%) were colonized by GBS.

The Sensitivity, Specificity, Positive predictive value and Negative predictive value of PCR was 54%, 88%, 76%, 72%, respectively.

Of the 42 examined GBS isolates, (76%) were susceptible to vancomycin, (57%) isolates were sensitive to penicillin, (50%) to erythromycin.

(48%), (31%) to tetracycline and clindamycin respectively.

There was no statistically significant association observed for GBS colonization with chronic disease, complications like (Previous abortion – delivery at <37weeks gestationpremature birth- intrauterine death – endometrtitis), and previous antibiotic intake (p <0.05).

Conclusion: Our results showed high prevalence of GBS in Gaza City, Palestine.

Despite the fact that PCR is well known for its high sensitivity, low sensitivity was obtained in this study.

Vancomycin was the most effective antibiotic against GBS isolates.

We, recommend a screening-based strategy to detect GBS in Palestinian pregnant women.

Main Subjects

Biology

No. of Pages

67

Table of Contents

• APA
• MLA
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Language

English

Data Type

Arab Theses

Record ID

BIM-900930