تشخيص الفنبلاستين و الفنكرستين في كالس نبات عين البزون Catharanthus roseus L. بجهاز كروماتوكرافيا السائل ذي الأداء العالي HPLC
Other Title(s)
Identification of vinblastine and vincristine in callus of catharanthus roseus l. by high performance liquid chromatography (hplc) apparatus
Joint Authors
باشي، بشار زكي أمين قصاب
المعماري، أيسر محمد سالم سعيد
الداودي، إياد جاجان
Source
Issue
Vol. 27, Issue 4 (31 Aug. 2018), pp.23-34, 12 p.
Publisher
University of Mosul College of Science
Publication Date
2018-08-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Parts of leaves, nodal explant, and internodes of Catharanthus roseus were cultured on solid MS medium supplemented with 30 gm / L sucrose, 6 mg / L agar with addition BA at 0.0, 0.1, 0.2, 0.3, 0.5 mg/ L with 0.75 mg /L 2,4-D for callus initiation and growth.
Vinblastine and Vincristine were identified in these calli by using High Performance Liquid Chromatography.(HPLC).
Data refers: highest fresh weight of callus and highest protein percentage 1.907 gm, 14.47% were achieved respectively from cultured parts of leaves on MS medium supplemented with 0.3 mg/ L BA with 0.75 mg/L 2,4-D.
cultured nodal explant on MS medium supplemented with 0.75 mg/ L 2, 4-D produced highest fresh weight of callus and highest protein percentage 0.496 gm, 9.15% respectively, culture internodes explant on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D produced highest fresh weight of callus and highest protein percentage 0.717 gm, 11.90 % respectively.
The callus produced from cultured parts of leave on MS medium supplemented with 0.3 mg/ L BA and 0.75 mg/L 2,4-D gave highest absorption value for Vinblastine at retention time (13.536) with detected area (255647).
highest detected area was (975021) at retention time (18.886) for Vincristine assessed from callus produced from cultured parts of leave on MS medium supplemented with 0.75 mg/L 2,4-D, callus produced from cultured nodes on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D gave highest detected area for Vinblastine (318395) at retention time (12.809) but culture nodes on MS medium supplemented with 0.3 mg/ L BA with 0.75 mg/L 2,4-D gave highest detected area (577979) for Vincristine at retention time (18.139), callus produced from cultured internodes on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D gave highest absorption value for Vinblastine and Vincristine (1892650) and (964402) at retention time (13.632) (18.309) respectively.
American Psychological Association (APA)
المعماري، أيسر محمد سالم سعيد وباشي، بشار زكي أمين قصاب والداودي، إياد جاجان. 2018. تشخيص الفنبلاستين و الفنكرستين في كالس نبات عين البزون Catharanthus roseus L. بجهاز كروماتوكرافيا السائل ذي الأداء العالي HPLC. مجلة علوم الرافدين،مج. 27، ع. 4، ص ص. 23-34.
https://search.emarefa.net/detail/BIM-901137
Modern Language Association (MLA)
المعماري، أيسر محمد سالم سعيد....[و آخرون]. تشخيص الفنبلاستين و الفنكرستين في كالس نبات عين البزون Catharanthus roseus L. بجهاز كروماتوكرافيا السائل ذي الأداء العالي HPLC. مجلة علوم الرافدين مج. 27، ع. 4 (2018)، ص ص. 23-34.
https://search.emarefa.net/detail/BIM-901137
American Medical Association (AMA)
المعماري، أيسر محمد سالم سعيد وباشي، بشار زكي أمين قصاب والداودي، إياد جاجان. تشخيص الفنبلاستين و الفنكرستين في كالس نبات عين البزون Catharanthus roseus L. بجهاز كروماتوكرافيا السائل ذي الأداء العالي HPLC. مجلة علوم الرافدين. 2018. مج. 27، ع. 4، ص ص. 23-34.
https://search.emarefa.net/detail/BIM-901137
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 33-34
Record ID
BIM-901137