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Partial purification and study of osteocalcin from blood of human and rats
Other Title(s)
تنقية جزئية و دراسة الاوستيوكالسين من دم الإنسان و الجرذان
Joint Authors
Allush, Thikra A.
Abd Allah, Liqa'a S.
Source
Issue
Vol. 27, Issue 4 (31 Aug. 2018), pp.19-28, 10 p.
Publisher
University of Mosul College of Science
Publication Date
2018-08-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
This study is concerned with attempts to isolate and purify osteocalcin from healthy human blood plasma and healthy rats blood serum using different biochemical techniques.
This included precipitation by cold acetone and gel filtration chromatography.
It was found that only the second peak (peak B) from human and rats had concentration of osteocalcin, and showed that fold of purification of isolated osteocalcin 57 and 43 for human and rats respectively.
The results obtained from electrophoresis showed that one single band for isolated osteocalcin (peak B) from human and rats, and from high performance liquid chromatography showed that there was a good identical in retention time between the standard and isolated osteocalcin (peak B) from human and rats.
The comparative molecular weight of partially purified osteocalcin from human plasma and rats serum was (6011.73 ± 400 Da) and (5248.07 ± 400 Da) respectively using gel filtration chromatography, and (5807.64 Da) and (4786.30 Da) respectively using electrophoresis technique.
American Psychological Association (APA)
Allush, Thikra A.& Abd Allah, Liqa'a S.. 2018. Partial purification and study of osteocalcin from blood of human and rats. Rafidain Journal of Science،Vol. 27, no. 4, pp.19-28.
https://search.emarefa.net/detail/BIM-901227
Modern Language Association (MLA)
Allush, Thikra A.& Abd Allah, Liqa'a S.. Partial purification and study of osteocalcin from blood of human and rats. Rafidain Journal of Science Vol. 27, no. 4 (2018), pp.19-28.
https://search.emarefa.net/detail/BIM-901227
American Medical Association (AMA)
Allush, Thikra A.& Abd Allah, Liqa'a S.. Partial purification and study of osteocalcin from blood of human and rats. Rafidain Journal of Science. 2018. Vol. 27, no. 4, pp.19-28.
https://search.emarefa.net/detail/BIM-901227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 27-28
Record ID
BIM-901227