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High-resolution melting curve (HRM) analysis in genotypic discrimination of cryptosporidium isolates from stool of Egyptian children
Joint Authors
al-Badri, Ayman A.
Khatir, Muna M.
Ghallab, Marwah M. I.
Tyler, Kevin M.
Bu Zayd, Maha
Abd al-Majid, Azzah Kamal
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 48, Issue 3 (31 Dec. 2018), pp.557-562, 6 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2018-12-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Topics
Abstract EN
Cryptosporidiosis is a recognized child infectious killer and the second cause of diarrheal disease and death in infants.
Assessing Cryptosporidium spp.
genetic diversity is a real goal to elucidate its transmission dynamics and to design preventive measures in absence of effective treatment.
Cryptosporidium isolates in stool of Egyptian children were detected using Acid Fast (AF) staining, copro-nPCR/RFLP assay and real time PCR high-resolution melting (HRM) curve analysis assay.
Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms, attending the outpatient clinic of Abu El Reesh hospital, Kasr Al-Ainy School of Medicine, Cairo University.
Two genotypes C.
hominis and C.
parvum were identified in 43 isolates from Egyptian children by copro-nPCR targeting COWP gene and HRM assay.
Real time PCR HRM curve analysis, a closed-tube genotyping method, targeting ITS-2 gene confirmed the results of copro-nPCR/RFLP.
It is simple, rapid, has more sample throughput, analysis capacities and data storage with less carry-over contamination and cost.
American Psychological Association (APA)
Ghallab, Marwah M. I.& Khatir, Muna M.& Tyler, Kevin M.& Bu Zayd, Maha& Abd al-Majid, Azzah Kamal& al-Badri, Ayman A.. 2018. High-resolution melting curve (HRM) analysis in genotypic discrimination of cryptosporidium isolates from stool of Egyptian children. Journal of the Egyptian Society of Parasitology،Vol. 48, no. 3, pp.557-562.
https://search.emarefa.net/detail/BIM-901247
Modern Language Association (MLA)
Ghallab, Marwah M. I.…[et al.]. High-resolution melting curve (HRM) analysis in genotypic discrimination of cryptosporidium isolates from stool of Egyptian children. Journal of the Egyptian Society of Parasitology Vol. 48, no. 3 (Dec. 2018), pp.557-562.
https://search.emarefa.net/detail/BIM-901247
American Medical Association (AMA)
Ghallab, Marwah M. I.& Khatir, Muna M.& Tyler, Kevin M.& Bu Zayd, Maha& Abd al-Majid, Azzah Kamal& al-Badri, Ayman A.. High-resolution melting curve (HRM) analysis in genotypic discrimination of cryptosporidium isolates from stool of Egyptian children. Journal of the Egyptian Society of Parasitology. 2018. Vol. 48, no. 3, pp.557-562.
https://search.emarefa.net/detail/BIM-901247
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 562
Record ID
BIM-901247