The protective role of N-acetyl cysteine and vitamin C against atrazine-induced dopaminergic neurodegeneration in N27 cells
Joint Authors
Inayah, Sabah Husayn
al-Awwadi, Afrah Abd Maqtuf
al-Atiyyah, Ahmad Khassaf
Source
Issue
Vol. 6, Issue 4 (30 Jun. 2018), pp.54-61, 8 p.
Publisher
University of Thi-Qar College of Science
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Background: Atrazine (ATZ)has been associated with its capability to interact and damage DNA in neuronal cells.
However, the molecular mechanisms of ATZ are not fully understood.
In vitro and In vivo studies indicated that atrazine induces cytotoxicity and oxidative stress in human and animals.
This study sought to determine the role of two antioxidants against the toxic impact of atrazine.
Main Methods: N27 cell line pretreated to N-acetyl Cysteine NAC or Ascorbic acid AA alone or in combination before 24h atrazine exposure.
Multiple intercellular antioxidant parameters measured such as (GSH, GSSG, MDA).
Genotoxicity represented by single gel electrophoreses assay (comet assay).
Results: the outcomes of this work demonstrated that higher protection against atrazine exposure at combination of NAC and AA comparing to the single exposure to each.
The antioxidant acted in synergistic way to rescue cells from reactive oxygen species generated from atrazine exposure.
DNA breaking or damage also prepared in better way in combination exposure comparing to the single exposure.
Conclusion: the evidence of atrazine increase the oxidative damage have been approved by enhancing defense system to increase cell availability and DNA repair.
While antioxidant (NAC&AA) prevent oxidative damage that causes by atrazine via increase cell viabilities and DNA fix.
American Psychological Association (APA)
Inayah, Sabah Husayn& al-Awwadi, Afrah Abd Maqtuf& al-Atiyyah, Ahmad Khassaf. 2018. The protective role of N-acetyl cysteine and vitamin C against atrazine-induced dopaminergic neurodegeneration in N27 cells. Journal of Thi-Qar Science،Vol. 6, no. 4, pp.54-61.
https://search.emarefa.net/detail/BIM-901470
Modern Language Association (MLA)
Inayah, Sabah Husayn…[et al.]. The protective role of N-acetyl cysteine and vitamin C against atrazine-induced dopaminergic neurodegeneration in N27 cells. Journal of Thi-Qar Science Vol. 6, no. 4 (Jun. 2018), pp.54-61.
https://search.emarefa.net/detail/BIM-901470
American Medical Association (AMA)
Inayah, Sabah Husayn& al-Awwadi, Afrah Abd Maqtuf& al-Atiyyah, Ahmad Khassaf. The protective role of N-acetyl cysteine and vitamin C against atrazine-induced dopaminergic neurodegeneration in N27 cells. Journal of Thi-Qar Science. 2018. Vol. 6, no. 4, pp.54-61.
https://search.emarefa.net/detail/BIM-901470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 59-61
Record ID
BIM-901470