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Spectrophotometric methods for determination of metronidazole in pharmaceutical formulations
Joint Authors
Hasan, Shlair H.
Abd Allah, Nirmin S.
Hasan, Midya A.
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 30, Issue 5 (31 Oct. 2018), pp.89-100, 12 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2018-10-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Two new, simple and accurate spectrophotometric methods for determination of metronidazole (MET) have been proposed.
The methods are based on the reduction of metronidazole with iron metal and hydrochloric acid then the subsequent of the formed reduction product which contains primary amino (NH2) group coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) reagent to form a purple colored chromogen that measured spectrophotometrically at 546 nm (Method A).
The method B depends on the reduction of tungstate and/or molybdate in Folin-Ciocaleteu reagent (FCR) by reduced metronidazole in alkaline medium to form a blue colored chromogen, which measured spectrophotometrically at 725 nm.
Under optimized reaction condition, the reaction of reduced metronidazole with both NBD-Cl and FCR get a good linearity in the concentration range of 0.3-30 and 0.5-40 μg/ ml for NBD-Cl and FCR, respectively.
The limits of detection and quantitation were 0.16 and 0.56 μg/ml for method A; while for method B were 0.05 and 0.18 μg/ml, respectively.
The accuracy and precision of the proposed methods were satisfactory.
The proposed methods were simple, rapid and successfully applied for analysis of MET in its pharmaceutical formulations.
American Psychological Association (APA)
Hasan, Shlair H.& Abd Allah, Nirmin S.& Hasan, Midya A.. 2018. Spectrophotometric methods for determination of metronidazole in pharmaceutical formulations. ZANCO Journal of Pure and Applied Sciences،Vol. 30, no. 5, pp.89-100.
https://search.emarefa.net/detail/BIM-901613
Modern Language Association (MLA)
Hasan, Shlair H.…[et al.]. Spectrophotometric methods for determination of metronidazole in pharmaceutical formulations. ZANCO Journal of Pure and Applied Sciences Vol. 30, no. 5 (2018), pp.89-100.
https://search.emarefa.net/detail/BIM-901613
American Medical Association (AMA)
Hasan, Shlair H.& Abd Allah, Nirmin S.& Hasan, Midya A.. Spectrophotometric methods for determination of metronidazole in pharmaceutical formulations. ZANCO Journal of Pure and Applied Sciences. 2018. Vol. 30, no. 5, pp.89-100.
https://search.emarefa.net/detail/BIM-901613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 99-100
Record ID
BIM-901613