Detection of some virulence factors with biochemical investigation of Listeria monocytogenes associated with human
Joint Authors
Hassuni, Adil Abid
Fadil, Ala Abbas
Source
al-Kufa University Journal for Biology
Issue
Vol. 10, Issue 1 (30 Apr. 2018), pp.113-119, 7 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2018-04-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
This study was aimed to detect different virulence genes that might be present in the listeria monocytogenes.
50 clinical samples were gathered from patients with different sites like (stool, urine and blood).
though, only (22) isolates were contained l.monocytogenes using conventional and traditional techniques.
strains were isolated with the following approach 8 (36%), 6 (27%), and 8 (36%) from human intestine (stool), urine, and blood samples respectively.
virulence genes were detected by using specific pcr primers which include (iap, hly andinl a).
the results indicated that four isolates of l.
monocytogenes possessed (hly) gene, two from urine strains 2:6 (33%), one from stool 1:8 (13%) and one from blood 1:8 (13%).
in addition to that, only three isolates of inlagave positive amplification where1:8(13%) from stool 1:6(17) from urine and 1:8 (13%) from blood.
moreover, six strains gave positive amplification for japgene where 3:8(38%) from stool and 2:6 (25%) from urine, and one fromblood1:8(13%).
American Psychological Association (APA)
Hassuni, Adil Abid& Fadil, Ala Abbas. 2018. Detection of some virulence factors with biochemical investigation of Listeria monocytogenes associated with human. al-Kufa University Journal for Biology،Vol. 10, no. 1, pp.113-119.
https://search.emarefa.net/detail/BIM-901867
Modern Language Association (MLA)
Hassuni, Adil Abid& Fadil, Ala Abbas. Detection of some virulence factors with biochemical investigation of Listeria monocytogenes associated with human. al-Kufa University Journal for Biology Vol. 10, no. 1 (2018), pp.113-119.
https://search.emarefa.net/detail/BIM-901867
American Medical Association (AMA)
Hassuni, Adil Abid& Fadil, Ala Abbas. Detection of some virulence factors with biochemical investigation of Listeria monocytogenes associated with human. al-Kufa University Journal for Biology. 2018. Vol. 10, no. 1, pp.113-119.
https://search.emarefa.net/detail/BIM-901867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 118-119
Record ID
BIM-901867