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The antifibrotic effect of Zilla spinosa extracts targeting apoptosis in CCl4-induced liver damage in rats
Joint Authors
al-Tumi, Sayyid A.
Nada, Sumayyah A.
Shabanah, Marwah E.
Farraj, Abd al-Raziq H.
Imarah, Inayat A.
al-Shafi, Nirmin
Source
Journal of the Arab Society for Medical Research
Issue
Vol. 13, Issue 2 (31 Dec. 2018), pp.129-143, 15 p.
Publisher
Arab Society for Medical Research
Publication Date
2018-12-31
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background/aim Liver fibrosis and its end-stage cirrhosis are the main reasons of morbidity and mortality all over the world.
The current study aimed to evaluate the efficacy of Zilla spinosa (Z.
spinosa) on CCl4-induced liver fibrosis, apoptosis, and oxidative stresses in rats.
Materials and methods Extract of aerial part of Z.
spinosa was used in this study.
Thirty male Sprague? Dawley rats were enrolled in this study and divided into five groups (six each): group 1 served as control and groups 2–5 were treated with CCl4 (1 ml/ kg intraperitoneal twice a week for 8 weeks), where group 2 served as a control positive, group 3 received silymarin (50 mg/kg) daily, and groups 4 and 5 were administrated with Z.
spinosa (100 and 200 mg/kg, respectively) daily for 8 weeks.
At the end of each experiment, liver function tests were analyzed in serum, whereas malondialdehyde (MDA), Nitric oxide (NO), Glutathione (GSH), and hydroxyproline (HA) were analyzed in liver tissues.
Liver fibrosis was confirmed histopathologically, and collagen content, caspase-3, and α-smooth muscle actin (α-SMA) were assayed immunhistochemically.
Results Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, MDA, NO, and HA levels were increased (P<0.05), whereas total protein and GSH were decreased (P<0.05) in CCl4-administrated rats.
Histopathological results showed loss of lobular structure, fibrosis with expansion of portal tract by fibrous tissue together with inflammatory changes confined to portal tract and central vein, and intense centrilobular necrosis and remarkable fatty hydropic degeneration.
In addition, extensive accumulation of connective tissue, marked depletion of glycogen, strong expression of α-SMA, and increased of caspase-3 were found in CCl4-administrated rats.
Oral administration of Z.
spinosa at 100 or 200 mg/kg restored the normal levels of liver function parameters, MDA, NO and GSH; decreased HA; and reduced collagen, glycogen content, caspase-3, and α-SMA in liver tissue of rats.
The high dose of 200 mg/kg showed more potent effect than low dose of 100 mg/kg when compared with silymarin treatment group.
Conclusion The present study clarified that Z.
spinosa extract has antioxidant and antiapoptotic properties in CCl4-induced liver fibrosis in rats, and may be able to exert a therapeutic effect on developing hepatic fibrosis; moreover, high dose of 200 mg/kg appeared to be more potent than low dose (100 mg/kg).
American Psychological Association (APA)
Imarah, Inayat A.& al-Tumi, Sayyid A.& Shabanah, Marwah E.& Farraj, Abd al-Raziq H.& Nada, Sumayyah A.& al-Shafi, Nirmin. 2018. The antifibrotic effect of Zilla spinosa extracts targeting apoptosis in CCl4-induced liver damage in rats. Journal of the Arab Society for Medical Research،Vol. 13, no. 2, pp.129-143.
https://search.emarefa.net/detail/BIM-902213
Modern Language Association (MLA)
Imarah, Inayat A.…[et al.]. The antifibrotic effect of Zilla spinosa extracts targeting apoptosis in CCl4-induced liver damage in rats. Journal of the Arab Society for Medical Research Vol. 13, no. 2 (Jul. / Dec. 2018), pp.129-143.
https://search.emarefa.net/detail/BIM-902213
American Medical Association (AMA)
Imarah, Inayat A.& al-Tumi, Sayyid A.& Shabanah, Marwah E.& Farraj, Abd al-Raziq H.& Nada, Sumayyah A.& al-Shafi, Nirmin. The antifibrotic effect of Zilla spinosa extracts targeting apoptosis in CCl4-induced liver damage in rats. Journal of the Arab Society for Medical Research. 2018. Vol. 13, no. 2, pp.129-143.
https://search.emarefa.net/detail/BIM-902213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 141-143
Record ID
BIM-902213