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The role of n-acetylcysteine supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of ex vivo murine haematopoietic stem progenitor cells
Other Title(s)
دور مكمالت الأسيتيل سيستني على مستويات الإجهاد التأكسدي و السمية الوراثية و قابلية عودة الانتساب للخلايا الجذعية المستخلصة حيويا خارج الجسم في الفئران أو الخلايا الجذرية
Joint Authors
Hamid, Zariyantey A.
Tan, Hui Y.
Chow, Paik W.
Harto, Khairul A. W.
Chan, Chin Yi
Muhammad, Jamal al-Din
Source
Sultan Qaboos University Medical Journal
Issue
Vol. 18, Issue 2 (31 May. 2018), pp.130-136, 7 p.
Publisher
Sultan Qaboos University College of Medicine and Health Sciences
Publication Date
2018-05-31
Country of Publication
Oman
No. of Pages
7
Main Subjects
Abstract EN
Objectives: The ex vivo maintenance of haematopoietic stem/progenitor cells (HSPCs) is crucial to ensure a sufficient supply of functional cells for research or therapeutic applications.
However, when exposed to reactive oxygen species (ROS) in a normoxic microenvironment, HSPCs exhibit genomic instability which may diminish their quantity and quality.
This study aimed to investigate the role of N-acetylcysteine (NAC) supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of murine haematopoietic stem/progenitor cells (HSPCs).
Methods: This study was carried out at the Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, between June 2016 and July 2017.
Bone marrow cells were isolated from nine mice and cultured in a growth medium.
Various concentrations of NAC between 0.125–2 µM were added to the culture for 48 hours; these cells were then compared to non-supplemented cells harvested from the remaining three mice as the control group.
A trypan blue exclusion test was performed to determine cell viability, while intracellular ROS levels and genotoxicity were determined by hydroethidine staining and comet assay, respectively.
The lineage commitment potential of erythroid, myeloid and pre-B-lymphoid progenitor cells was evaluated via colony-forming cell assay.
Results: NAC supplementation at 0.25, 0.5 and 2 µM significantly increased cell viability (P <0.050), while intracellular ROS levels significantly decreased at 0.25 and 0.5 µM (P <0.050).
Moreover, DNA damage was significantly reduced at all NAC concentrations (P <0.050).
Finally, the potential lineage commitment of the cells was not significantly affected by NAC supplementation (P >0.050).
Conclusion: The findings of this study indicate that NAC supplementation may potentially overcome the therapeutic limitations of ex vivo-maintained HSPCs.
American Psychological Association (APA)
Hamid, Zariyantey A.& Tan, Hui Y.& Chow, Paik W.& Harto, Khairul A. W.& Chan, Chin Yi& Muhammad, Jamal al-Din. 2018. The role of n-acetylcysteine supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of ex vivo murine haematopoietic stem progenitor cells. Sultan Qaboos University Medical Journal،Vol. 18, no. 2, pp.130-136.
https://search.emarefa.net/detail/BIM-902953
Modern Language Association (MLA)
Harto, Khairul A. W.…[et al.]. The role of n-acetylcysteine supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of ex vivo murine haematopoietic stem progenitor cells. Sultan Qaboos University Medical Journal Vol. 18, no. 2 (May. 2018), pp.130-136.
https://search.emarefa.net/detail/BIM-902953
American Medical Association (AMA)
Hamid, Zariyantey A.& Tan, Hui Y.& Chow, Paik W.& Harto, Khairul A. W.& Chan, Chin Yi& Muhammad, Jamal al-Din. The role of n-acetylcysteine supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of ex vivo murine haematopoietic stem progenitor cells. Sultan Qaboos University Medical Journal. 2018. Vol. 18, no. 2, pp.130-136.
https://search.emarefa.net/detail/BIM-902953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 135-136
Record ID
BIM-902953