Purification and characterization of produced cellulase enzyme by irradiated achromobacter spanius

Abstract EN

Achromobacter spanius was endophytic cellulase producing bacteria.

they were irradiated by gamma rays at doses from 0.5 – 5 kGy.

irradiated Achromobacter spanius increase extracellular cellulase activity in comparison with non-irradiated strain.

irradiated strain at dose 3.5 kGy was identified as higher cellulase enzyme activity than those of other doses.

the cellulase enzyme was purified by applying sephadex G-100 gel chromatography technique.

the highest activity of cellulase was obtained at enzyme volume and incubation period of 0.2 ml and 30 minutes respectively.

The optimum pH value was 7 and the optimum activity with temperature was 35 oC.

the enzyme showed the highest activity when carboxmethyl cellulose (CMC) used as substrate on reaction mixture.

according to thermal stability, the purified cellulase has high activity after 5 minutes when purified cellulase was incubated at 60 oc.

the purified cellulase, with a molecular weight (M.W.) of 32.5 kDa.