Gene expression of blaoxa-51-like and blaoxa-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter Baumannii and Acinetobacter Lowffii from urine samples
Other Title(s)
التعبير الجيني ل blaOXA-51-like و blaOXA-23 كاستجابة للمضادات الحيوية الحاوية على β-Lactam في بكتريا الراكدة البومانية Acinetobacter baumannii و الراكدة اللوفية Acinetobacter lowffii المعزولة سريريا
Author
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 4 (31 Aug. 2019), pp.1120-1137, 18 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-08-31
Country of Publication
Iraq
No. of Pages
18
Main Subjects
Topics
Abstract EN
In this study, two isolates A.
lowffii, and A.
baumannii were obtained clinically, and genomically identified by 16S rRNA assessment with the accession number (MH685113.1 and MH685112.1) for A.
lowffii and A.
baumannii respectively.
The sensitivity profile of the isolates was variable, and A.
baumannii was the most resistant strain towards a wide range of antibiotics, and it did not show any growth defect in the presence of β-lactam antibiotic, in comparison with A.
lowffii.
We identified that the blaOXA-23 gene was responsible for imipenem resistance in A.
baumannii, whereas, blaOXA-51-like was moderately confer resistance towards A.
lowffii which lack of blaOXA-23.
This was determined when the isolates were subjected to qRTPCR.
We identified that the blaOXA-23 gene was increased about 1-fold in the presence of imipenem, whereas, blaOXA-51-like did not increased in comparison to the control.
Bioinformatic analyses revealed that blaOXA-23 is located in the cytoplasm, and blaOXA-like 51 is located in the periplasm.
Our hypothesis suggests that blaOXA-23 gene have a major contribution in the outbreak of multidrug resistance Acinetobacter species.
American Psychological Association (APA)
Ali, Halah Haytham Muhammad. 2019. Gene expression of blaoxa-51-like and blaoxa-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter Baumannii and Acinetobacter Lowffii from urine samples. The Iraqi Journal of Agricultural Science،Vol. 50, no. 4, pp.1120-1137.
https://search.emarefa.net/detail/BIM-903842
Modern Language Association (MLA)
Ali, Halah Haytham Muhammad. Gene expression of blaoxa-51-like and blaoxa-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter Baumannii and Acinetobacter Lowffii from urine samples. The Iraqi Journal of Agricultural Science Vol. 50, no. 4 (2019), pp.1120-1137.
https://search.emarefa.net/detail/BIM-903842
American Medical Association (AMA)
Ali, Halah Haytham Muhammad. Gene expression of blaoxa-51-like and blaoxa-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter Baumannii and Acinetobacter Lowffii from urine samples. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 4, pp.1120-1137.
https://search.emarefa.net/detail/BIM-903842
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-903842