Suppressing the migration of human breast cancer cell line by targeting VAMP3 with miR-199a-3p

Abstract EN

Deregulation of microRNAs contributes to multiple processes in cancer growth and progression.

miR-199a-3p is decreased in highly metastatic breast cancer cells, MDA-MB-231, and its ectopic expression has a potent antimetastatic eff ect on these cells.

However, the mechanism by which miR-199a-3p mediates its antimetastatic function has yet to be elucidated.

Because miR-199a-3p reduces the expression levels of its target genes, it is likely to observe an inverse association between miR-199a-3p and its prometastatic target genes at the expression level.

The current work determines that the Vesicleassociated membrane protein 3 (VAMP3) expression is increased in highly metastatic breast cancer cells compared to less metastatic cells, Michigan Cancer Foundation-7.

The ectopic expression of miR199a-3p strongly inhibits VAMP3 Messenger RNA and protein in vitro.

Herein, it is confi rmed that two sites within the 3-untranslated sequence of VAMP3 Messenger RNA are actively targeted by miR-199a3p, discovering a new regulatory mechanism for VAMP3 expression.

Functional studies reveal that the suppression of VAMP3 contributes to miR-199a-3p antimetastatic eff ect, particularly cellular migration in vitro.

In conclusion, these results indicate that miR-199a-3p targeting of VAMP3 possesses a signifi cant potential impact in preventing or curing metastatic breast cancers