Suppressing the migration of human breast cancer cell line by targeting VAMP3 with miR-199a-3p
Other Title(s)
miR-199a-3p ينظم هجرة خلايا سرطان الثدي من خلال استهداف جين VAMP3
Joint Authors
Laqtum, Nuf Nasir
al-Qathmi, Khulud Mubarak
Source
Journal of King Abdulaziz University : Medical Sciences
Issue
Vol. 23, Issue 2 (30 Jun. 2016), pp.29-37, 9 p.
Publisher
King Abdulaziz University Scientific Publishing Center
Publication Date
2016-06-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Abstract EN
Deregulation of microRNAs contributes to multiple processes in cancer growth and progression.
miR-199a-3p is decreased in highly metastatic breast cancer cells, MDA-MB-231, and its ectopic expression has a potent antimetastatic eff ect on these cells.
However, the mechanism by which miR-199a-3p mediates its antimetastatic function has yet to be elucidated.
Because miR-199a-3p reduces the expression levels of its target genes, it is likely to observe an inverse association between miR-199a-3p and its prometastatic target genes at the expression level.
The current work determines that the Vesicleassociated membrane protein 3 (VAMP3) expression is increased in highly metastatic breast cancer cells compared to less metastatic cells, Michigan Cancer Foundation-7.
The ectopic expression of miR199a-3p strongly inhibits VAMP3 Messenger RNA and protein in vitro.
Herein, it is confi rmed that two sites within the 3-untranslated sequence of VAMP3 Messenger RNA are actively targeted by miR-199a3p, discovering a new regulatory mechanism for VAMP3 expression.
Functional studies reveal that the suppression of VAMP3 contributes to miR-199a-3p antimetastatic eff ect, particularly cellular migration in vitro.
In conclusion, these results indicate that miR-199a-3p targeting of VAMP3 possesses a signifi cant potential impact in preventing or curing metastatic breast cancers
American Psychological Association (APA)
Laqtum, Nuf Nasir& al-Qathmi, Khulud Mubarak. 2016. Suppressing the migration of human breast cancer cell line by targeting VAMP3 with miR-199a-3p. Journal of King Abdulaziz University : Medical Sciences،Vol. 23, no. 2, pp.29-37.
https://search.emarefa.net/detail/BIM-904036
Modern Language Association (MLA)
Laqtum, Nuf Nasir& al-Qathmi, Khulud Mubarak. Suppressing the migration of human breast cancer cell line by targeting VAMP3 with miR-199a-3p. Journal of King Abdulaziz University : Medical Sciences Vol. 23, no. 2 (Jun. 2016), pp.29-37.
https://search.emarefa.net/detail/BIM-904036
American Medical Association (AMA)
Laqtum, Nuf Nasir& al-Qathmi, Khulud Mubarak. Suppressing the migration of human breast cancer cell line by targeting VAMP3 with miR-199a-3p. Journal of King Abdulaziz University : Medical Sciences. 2016. Vol. 23, no. 2, pp.29-37.
https://search.emarefa.net/detail/BIM-904036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 35-36
Record ID
BIM-904036