Improve shoot proliferation of nodal segments of jojoba (simmondsia chinensis schneider)‎ genotypes by growth regulators combinations

Abstract EN

The effect of plant growth regulates on in vitro nodal segments of four jojoba genotypes were investigated.

Shoot initiation was performed using MS medium supplemented with BAP in combinations with NAA, IAA and 2,4- D.

The experiments were conducted using the completely Randomized design(CRD) and 4 replicates.

The results of in vitro nodal segments indicated that the culture medium without growth regulators (MS0), MS + 5mg/l-1 BAP, MS + 3mg/l-1 BAP + 5mg/l-1 IAA, MS + 5mg/l-1 BAP +7mg/l-1 NAA and MS + 7mg/l-1 BAP + 7mg/l-1 NAA significantly decreased number of days to sprouting, increased % of sprouted buds.

The culture medium MS + 7mg/l-1 BAP + 7mg/l-1 NAA produced the highest no.

of leaves/shoot, length of primary shoots and no.

of nodes/shoot.

Explants of the genotype ‘Hail-B’ registered the highest no.

of days for bud sprouting.

The genotypes ‘Hail-A‘ and ‘Al- Madinha’ produced the highest percentage of sprouted buds, no.

of leaves/shoot, length of primary shoots cm) and no.

of nodes/shoot.

The jojoba genotypes ‘Hail A’, ‘Al-Madinah’ and ‘Hada Al-Sham’ were the most useful genotypes for micropropagation using nodal segments.

The MS media supplemented with5mg/L-1 BAP alone or with 3mg/L-1 BAB and 5mg/L-1 IAA can be applied for jojoba micropropagation using nodal segments explants.