The effects of topical ketamine gel on saliva and serum level of interlukin-6 in patients with recurrent Aphthous ulceration

Abstract EN

To evaluate the effect of topical application of ketamine gel )0.5%( and gel alone)without ketamine( on salivary and serum level of human interlukin-6)IL-6 (in patient with recurrent aphthous ulceration )RAU( and compare the results with control group having no RAU.

Materials and methods: The investigation was carried out on)36( subjects with mean ± SD ages )25.11±6.31(years and different sex )20 males,16 females(.

These subjects were divided into three groups.

Group1: The study group consisted of )12(patients having minor RAU received topical ketamine gel )0.5%( applied on the lesion.

Group2: The placebo group consisted of )12(patients having minor RAU received topical gel alone.

Group3: The control group consisted of )12( healthy subjects having no RAU.Peripheral venous blood and saliva samples were collected from all subjects before treatment, and four days after treatment from group1and group2 only, to assess the levels of serum and salivary )IL-6( by Enzyme Linked Immuno Sorbent Assays )ELISA( method.

Patients were subjectively assessed for the intensity of pain which was recorded pre and four days post treatment in group1 and 2 by using Verbal Pain Scale )VPS(.

Data were analyzed using paired t-test, One way ANOVA and Willcoxon Signed Ranks test.

Results: The results showed that the mean of serum IL-6 concentrations were high in the study and placebo groups )18.49±5.4pg/ml( and )18.97± 7,47 pg/ml( respectively before treatment comparing with mean of serum IL-6 concentration of control group )9.63±2.77pg/ml(, and the mean of salivary IL-6 concentrations were high in the study and placebo groups )63.03±47.28pg/ml( and )62.25±45.14pg/ml( respectively before treatment comparing with mean of saliva IL-6 concentration of control group )13.1±5.08pg/ml(.

Treatment by topical ketamine gel (0.5%) had led to significant decrease in the means of serum and salivary IL-6 level in the study group to (11.31±4.49pg/ml) and (22.61±16.81pg/ml) respectively, whereas the means of serum and salivary IL-6 level were increased to (36.97±25.87pg/ml) and (85.63±67.29pg/ml) respectively 4 days after treatment by topical gel alone in placebo group.

The intensity of pain was significantly decreased from (3) in study and placebo groups to (0) and (1.33±1.3) respectively after 4 days of treatment.

Conclusions: Data denote that ketamine gel posses anti inflammatory effect by decrease the levels of serum and salivary IL-6 in patients with RAU that may reduce inflammation and pain after topical application.