تشخيص ثلاث سلالات جديدة تابعة لفيروس تجعد و اصفرار أوراق الطماطم البندورة (Tomato yellow leaf curl virus)‎ في العراق

Abstract EN

This study was conducted to identify and characterize three new strains of Tomato yellow leaf curl virus (Begomovirus, Geminiviridae) from three Iraqi provinces (Najaf, Babylon, and Al-Muthanna) using the polymerase chain reaction (PCR) technique and the nucleotide sequence analysis of amplified DNA.

Results showed that the evaluated virus strains were not previously recorded at the USA National Center for Biotechnology Information (NCBI).

All three virus strains characterized were registered at NCBI with GenBank accession numbers: KT961704, KT961705 and KT961706.

A Basic Local Alignment Search Tool (BLAST) was applied on all nucleotide sequences obtained from these three virus strains, showed a similarity level of 99% when compared with an Iraqi TYLCV isolate (GenBank accession no.: JQ354991.1).

Moreover, it was also found that the similarity level among the three characterized virus strains reached 99%.

Keywords: TYLCV, PCR, characterization, Iraq