Current status of watermelon chlorotic stunt virus (WmCSV) on some cucurbit plants (Cucurbitaceae) in Alahsa Region of Saudi Arabia
Other Title(s)
الوضع الحالي لفيروس اصفرار و تقزم البطيخ على بعض النباتات القرعية بمنطقة الأحساء بالمملكة العربية السعودية
Joint Authors
Rizq, Adil Abd al-Sabur
Sulayman, Ahmad Muhammad
al-Hudayb, Khalid Abd Allah
Source
Scientific Journal of King Faisal University : Basic and Applied Sciences
Issue
Vol. 19, Issue 2 (31 Dec. 2018), pp.37-45, 9 p.
Publisher
Publication Date
2018-12-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Abstract EN
Cucurbit crops are widely grown and consumed in large quantities in Saudi Arabia and Middle East countries.
Viral diseases are among the most limiting factors that affect the production of cucurbit crops around the world.
The infection percentage of watermelon chlorotic stunt disease and molecular characterization of Saudi isolate of WmCSV are described in this study.
Symptomatic leaf samples of zucchini (Cucurbita pepo), Cucumber (Cucumis sativus) and melon (Cucumis melo L) plants were tested for WmCSV and squash leaf curl virus (SLCV) infection serologically using the double antibody sandwich- enzyme-linked immunosorbent assay (DAS-ELISA) test and polymerase chain reaction (PCR) using degenerate and specific overlapping primers for WmCSV to amplify 2108 nucleotides of DNA-A.
The data of DAS-ELISA test showed that 69% of all tested zucchini samples were infected with WmCSV, the percentage of infection increased to 72.7% when using PCR test.
All tested samples of other cucumber and melon tested negative for WmCSV while SLCV was not detected in all tested samples.
The obtained sequence analysis data demonstrated that the isolated WmCSV shared high nucleotide identity 98% with other isolates of Oman.
The nucleotide sequences of WmCSV obtained in this study was submitted to the GenBank (International Nucleotide Sequence Database Collaboration) under accession number KC876038.
American Psychological Association (APA)
al-Hudayb, Khalid Abd Allah& Rizq, Adil Abd al-Sabur& Sulayman, Ahmad Muhammad. 2018. Current status of watermelon chlorotic stunt virus (WmCSV) on some cucurbit plants (Cucurbitaceae) in Alahsa Region of Saudi Arabia. Scientific Journal of King Faisal University : Basic and Applied Sciences،Vol. 19, no. 2, pp.37-45.
https://search.emarefa.net/detail/BIM-908239
Modern Language Association (MLA)
al-Hudayb, Khalid Abd Allah…[et al.]. Current status of watermelon chlorotic stunt virus (WmCSV) on some cucurbit plants (Cucurbitaceae) in Alahsa Region of Saudi Arabia. Scientific Journal of King Faisal University : Basic and Applied Sciences Vol. 19, no. 2 (Dec. 2018), pp.37-45.
https://search.emarefa.net/detail/BIM-908239
American Medical Association (AMA)
al-Hudayb, Khalid Abd Allah& Rizq, Adil Abd al-Sabur& Sulayman, Ahmad Muhammad. Current status of watermelon chlorotic stunt virus (WmCSV) on some cucurbit plants (Cucurbitaceae) in Alahsa Region of Saudi Arabia. Scientific Journal of King Faisal University : Basic and Applied Sciences. 2018. Vol. 19, no. 2, pp.37-45.
https://search.emarefa.net/detail/BIM-908239
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-908239