Henotypic and genotypic characterization of pseudomonas aeruginosa recovered from kidney lesions of broiler chickens

Abstract EN

his work was planned to investigate the prevalence of P.

aeruginosa in broiler chickens kidneys suffered from naked eye pathological lesions.

Out of 240 examined kidneys samples, 25 isolates (10.4%) of P.

aeruginosa were recovered.

Antimicrobial sensitivity pattern against 14 different antimicrobials comprising nine groups proved that isolates were resistant to most of the used antimicrobial agents.

Phenotypic detection of extended spectrum beta-lactamases (ESBLs) producing isolates using modified double disk diffusion test revealed that, ten (40%) were ESBLs producers.

Genotypically using PCR, three ESBLs genes (TEM, Ctx-M and Shv) and three virulence genes (lasI, Tox A and Plc H) were detected with different percentages.

Cinnamon and carvacrol oils were found bactericidal for P.

aeruginosa at concentrations of 4% and 0.4% respectively