Ameliorative effect of spirulina platensis against diclofenac sodium-induced hepatotoxicity in broilers : hematological, biochemical, antioxidant, immunological and histopathological study
Other Title(s)
التأثير التحسني للسبيرولينا بلاتنسيس ضد السمية الكبدية الناجمة عن ديكلوفيناك الصوديوم في دجاج التسمين: دراسات دموية، و بيوكيميائية، و مضادات الأكسدة، و مناعية، و باثولوجية
Joint Authors
Mukhbatli, Abd Allah Ahmad
Ghazi, Imad Wadid
Abd al-Hadi, Dua Husni
Walid, Abduh
Abd al-Munim, Marwah Abd al-Wahhab
Source
Assiut Veterinary Medical Journal
Issue
Vol. 64, Issue 156 (31 Jan. 2018), pp.146-177, 32 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2018-01-31
Country of Publication
Egypt
No. of Pages
32
Main Subjects
Abstract EN
Spirulina is one of the most commonly used dietary supplements in humans, aquaculture, aquarium, and recently in poultry industries and gained more attention to be used as a nutritional and therapeutic strategy.
The present study has been carried out to investigate the effect of oral supplementation of Spirulina platensis in alleviating the hepatotoxic effect of diclofenac sodium through evaluating the hematological, biochemical, antioxidant parameters as well as immunological and histopathological studies.
One hundred broiler chicks were divided into four groups (25 per each).
The first group was fed on basal diet and treated with propylene glycol (2.5 mg/kg, i.m) (Control negative).
The second group was fed on basal diet and injected with diclofenac sodium at dose (2.5 mg/kg.b.wt., i.m) (Control positive).
The third group was injected with diclofenac sodium and then concurrently supplemented with Spirulina at dose (10 gm/kg in their diet).
The fourth group was fed on Spirulina two weeks before injection of diclofenac sodium at dose (2.5 mg/kg, i.m).
Haematological data revealed that diclofenactreated chicks showed normocytic normochromic anemia, significant leucopenia and decrease the absolute number of heterophils and lymphocytes.
Significant increase in serum biochemical parameters related to liver injury such as AST and ALP, total cholesterol and significant decrease in the levels of serum total proteins and albumin.
Significant increase in uric acid level was also noticed.
Furthermore, significant increase of oxidative damage biomarkers such as MDA and NO and significant decrease of the antioxidant enzymes as CAT, SOD and TAC.
Moreover, diclofenac sodium has immunosuppressive effect through depressed phagocytic activity, phagocytic index and marked decrease in the titer of antibodies formed in chicks against Newcastle disease.
Histopathological findings marked hepatic tissue damage due to gouty tophi lesions accompanied with presence of multifocal necrotic areas.
Oral supplementation of Spirulina markedly decrease the histopathological alteration on preventive basis.
Therefore, Spirulina supplementation markedly alleviated the deteriorating effects of diclofenac sodium-induced hepatotoxicity which mimics the gouty lesions in birds either on therapeutic or preventive regimes.
American Psychological Association (APA)
Mukhbatli, Abd Allah Ahmad& Ghazi, Imad Wadid& Abd al-Hadi, Dua Husni& Walid, Abduh& Abd al-Munim, Marwah Abd al-Wahhab. 2018. Ameliorative effect of spirulina platensis against diclofenac sodium-induced hepatotoxicity in broilers : hematological, biochemical, antioxidant, immunological and histopathological study. Assiut Veterinary Medical Journal،Vol. 64, no. 156, pp.146-177.
https://search.emarefa.net/detail/BIM-909129
Modern Language Association (MLA)
Mukhbatli, Abd Allah Ahmad…[et al.]. Ameliorative effect of spirulina platensis against diclofenac sodium-induced hepatotoxicity in broilers : hematological, biochemical, antioxidant, immunological and histopathological study. Assiut Veterinary Medical Journal Vol. 64, no. 156 (Jan. 2018), pp.146-177.
https://search.emarefa.net/detail/BIM-909129
American Medical Association (AMA)
Mukhbatli, Abd Allah Ahmad& Ghazi, Imad Wadid& Abd al-Hadi, Dua Husni& Walid, Abduh& Abd al-Munim, Marwah Abd al-Wahhab. Ameliorative effect of spirulina platensis against diclofenac sodium-induced hepatotoxicity in broilers : hematological, biochemical, antioxidant, immunological and histopathological study. Assiut Veterinary Medical Journal. 2018. Vol. 64, no. 156, pp.146-177.
https://search.emarefa.net/detail/BIM-909129
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-909129