Epidemiological studies and molecular characterization of clostridium perfringens in small rumeniant at el-Behera Governorate, Egypt

Abstract EN

Epidemiological factors associated with clostridium infection among sheep and goats.

Through the collection of samples from suspected cases of sheep and goat, anaerobic isolation and identification of C.

perfringens by using classical methods and application of multiplex PCR for molecular characterization of isolates.

A total of 104 samples were collected from intestine, liver, kidney and spleen from sheep and goat either those showing signs of enterotoxaemia or suddenly dead from different farms and small holders at El- Behera Governorate for anaerobic bacteriological examination.

Our study concluded that, the C.

perfringens causes high mortality in sheep and goat as a result secretion of Alpha, Beta and Epislon toxins.

Molecular characterization of C.

perfringens by Multiplex PCR characterize C.

perfringenstype"A" (alpha toxin) which gave a characteristic band at 402 bp.