Phorate triggers oxidative stress and mitochondrial dysfunction to enhance micronuclei generation and DNA damage in human lymphocytes
Joint Authors
Saquib, Quaiser
Ansari, Sabihah Mahmud
Wahhab, Radwan
Faysal, Muhammad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1411-1417, 7 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Herein, we studied phorate for its toxicological effects in human lymphocytes.
Phorate treatment for 3 h has induced significant increase in the lymphocytic DNA damage.
Compared to control, comet data from highest concentration of phorate (1000 µM) showed 8.03-fold increase in the Olive tail moment (OTM).
Cytokinesis blocked micronucleus (CBMN) assay revealed 6.4-fold increase in binucleated micronucleated (BNMN) cells following the exposure with phorate (200 µM) for 24 h.
The nuclear division index (NDI) in phorate (200 µM) treated cells reduced to 1.8 vis-à-vis control cells showed NDI of 1.94.
Comparative to untreated control, 60.43% greater DCF fluorescence was quantitated in lymphocytes treated with phorate (500 µM), affirming reactive oxygen species (ROS) generation and oxidative stress.
Flow cytometric data of phorate (200 µM) treated lymphocytes showed 81.77% decline in the fluorescence of rhodamine 123 (Rh123) dye, confirming the perturbation of mitochondrial membrane potential (ΔΨm).
Calf thymus DNA (ct-DNA) treated with phorate (1000 µM) exhibited 2.3-fold higher 8-Hydroxy-2′-deoxyguanosine (8-oxodG) DNA adduct formation, signified the oxidative DNA damage.
The alkaline unwinding assay revealed 4.0 and 6.5 ct-DNA strand breaks when treated to phorate and phorate-Cu (II) complex.
Overall, the data unequivocally suggests the cyto- and genotoxic potential of phorate in human lymphocytes, which may induce comparable toxicological consequences in persons occupationally or non-occupationally exposed to insecticide phorate.
American Psychological Association (APA)
Saquib, Quaiser& Faysal, Muhammad& Ansari, Sabihah Mahmud& Wahhab, Radwan. 2019. Phorate triggers oxidative stress and mitochondrial dysfunction to enhance micronuclei generation and DNA damage in human lymphocytes. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1411-1417.
https://search.emarefa.net/detail/BIM-909990
Modern Language Association (MLA)
Saquib, Quaiser…[et al.]. Phorate triggers oxidative stress and mitochondrial dysfunction to enhance micronuclei generation and DNA damage in human lymphocytes. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1411-1417.
https://search.emarefa.net/detail/BIM-909990
American Medical Association (AMA)
Saquib, Quaiser& Faysal, Muhammad& Ansari, Sabihah Mahmud& Wahhab, Radwan. Phorate triggers oxidative stress and mitochondrial dysfunction to enhance micronuclei generation and DNA damage in human lymphocytes. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1411-1417.
https://search.emarefa.net/detail/BIM-909990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1417
Record ID
BIM-909990