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Nosema ceranae infection in honeybee samples from Tuscanian archipelago (Central Italy) investigated by two qPCR methods
Joint Authors
Cilia, Giovanni
Sagona, Simona
Giusti, Matteo
Santos, Pedro Emanuel Jarmela Dos
Nanetti, Antonio
Felicioli, Antonio
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1553-1556, 4 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
Nosema apis and Nosema ceranae are microsporidian parasite worldwide spread causing an emerging infectious disease of European honeybee Apis mellifera.
The Nosema presence was deeply investigated in several countries but low information are presents about islands.
In this investigation was evaluated the presence N.
ceranae and N.
apis in apiaries located in Tuscanian Archipelago islands (Central Italy).
For N.
ceranae detection, two different Real-Time PCR (qPCR) methods, the 16S rRNA and Hsp70 gene amplification qPCR, were performed on honey bee samples; while, for N.
apis only the 16S rRNA qPCR amplification was performed.
On all islands, only N.
ceranae was present, while N.
apis was not found in the samples.
The two qPCR showed significant difference (p < 0.040) in N.
ceranae spores quantification.
The single-copy Hsp70 gene method qPCR assay systematically detected a lower amount of N.
ceranae copies compared to the multi-copy 16S rRNA gene method.
American Psychological Association (APA)
Cilia, Giovanni& Sagona, Simona& Giusti, Matteo& Santos, Pedro Emanuel Jarmela Dos& Nanetti, Antonio& Felicioli, Antonio. 2019. Nosema ceranae infection in honeybee samples from Tuscanian archipelago (Central Italy) investigated by two qPCR methods. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1553-1556.
https://search.emarefa.net/detail/BIM-910173
Modern Language Association (MLA)
Cilia, Giovanni…[et al.]. Nosema ceranae infection in honeybee samples from Tuscanian archipelago (Central Italy) investigated by two qPCR methods. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1553-1556.
https://search.emarefa.net/detail/BIM-910173
American Medical Association (AMA)
Cilia, Giovanni& Sagona, Simona& Giusti, Matteo& Santos, Pedro Emanuel Jarmela Dos& Nanetti, Antonio& Felicioli, Antonio. Nosema ceranae infection in honeybee samples from Tuscanian archipelago (Central Italy) investigated by two qPCR methods. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1553-1556.
https://search.emarefa.net/detail/BIM-910173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1556
Record ID
BIM-910173