Stenotrophomonas maltophilia Gd2 : a potential and novel isolate for fibrinolytic enzyme production
Joint Authors
Prakasham, R. S.
Khursade, Parag S.
Galande, Sneha H.
Krishna, P. Shiva
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1567-1575, 9 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
The bacterium with an ability to produce extracellular fibrinolytic protease was isolated and identified as Stenotrophomonas maltophilia Gd2 based on ribotyping.
The in-vitro fibrinolytic profile of this enzyme depicted 73% of fibrin clot dissolution within 4 h.
Fibrinolytic enzyme yield influenced by different physiological (incubation time, temperature, agitation and pH), nutritional (macronutrients such as carbon and nitrogen sources) and biological (inoculums age and inoculums concentration) parameters of fermentation which were optimized based on one-factor-at-a-time (OFAT) approach.
The enzyme yield improved from 886 to 1795 FU ml−1 upon OFAT; optimized conditions include temperature – 33 °C, pH – 8.0, incubation time – 36 h, agitation – 150 RPM, 3% v/v inoculums and age of inoculum – 18 h.
Further optimization of enzyme production was achieved with implementation of Plackett-Burman media designing where
American Psychological Association (APA)
Khursade, Parag S.& Galande, Sneha H.& Krishna, P. Shiva& Prakasham, R. S.. 2019. Stenotrophomonas maltophilia Gd2 : a potential and novel isolate for fibrinolytic enzyme production. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1567-1575.
https://search.emarefa.net/detail/BIM-910176
Modern Language Association (MLA)
Khursade, Parag S.…[et al.]. Stenotrophomonas maltophilia Gd2 : a potential and novel isolate for fibrinolytic enzyme production. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1567-1575.
https://search.emarefa.net/detail/BIM-910176
American Medical Association (AMA)
Khursade, Parag S.& Galande, Sneha H.& Krishna, P. Shiva& Prakasham, R. S.. Stenotrophomonas maltophilia Gd2 : a potential and novel isolate for fibrinolytic enzyme production. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1567-1575.
https://search.emarefa.net/detail/BIM-910176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1575
Record ID
BIM-910176