Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer
Joint Authors
Chen, Bo
Qiao, Xiaofeng
Li, Daguang
Zhang, Lingmin
Yuan, Cuiling
Lin, Hua
Yang, Qing
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 8 (31 Dec. 2019), pp.2048-2051, 4 p.
Publisher
Publication Date
2019-12-31
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
Objective: To study the expression of three genes IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer; analyse the differences in their expression in laryngeal cancer and adjacent tissues; by using pEGFP-N1-IL21 and pGPU/GFP/Neo-FBXL20 expression vectors, to analyse the characteristics in their expression in laryngeal cancer cells outside the body as well as the associations among them.
Methods: The expression of the three genes in laryngeal cancer and adjacent tissues from 30 cases and in normal laryngeal tissues from 20 healthy persons was detected with the RT-PCR; laryngeal cancer cell line (HEp-2 cells) transfection was also performed with the pEGFP-N1-IL21 and pGPU/GFP/Neo-FBXL20 expression vectors we constructed, to detect the mRNA expression of the three genes.
Cell proliferation, apoptosis and cell cycle were measured by the MTT assay.
Results: The results of RT-PCR showed that the expression of IL-21 and FBXL20 was up-regulated in laryngeal cancer, while the expression of tumour suppressor gene PTEN was significantly decreased (p < 0.01).
In HEp-2 cells transfected with pGPU/GFP/Neo-IL-21 and pGPU/GFP/Neo-FBXL20 expression vectors, the mRNA expression of PTEN was restored to some extent (p < 0.05); in addition, the ability of HEp-2 cells in proliferation and invasion was also reduced.
Conclusions: IL-21 and FBXL20 genes are important in the occurrence and development of laryngeal cancer; the expression of PTEN gene can suppress laryngeal cancer, and there’s a certain association among IL-21, FBXL20 and PTEN.
American Psychological Association (APA)
Yang, Qing& Qiao, Xiaofeng& Li, Daguang& Chen, Bo& Zhang, Lingmin& Yuan, Cuiling…[et al.]. 2019. Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer. Saudi Journal of Biological Sciences،Vol. 26, no. 8, pp.2048-2051.
https://search.emarefa.net/detail/BIM-910228
Modern Language Association (MLA)
Yang, Qing…[et al.]. Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer. Saudi Journal of Biological Sciences Vol. 26, no. 8 (Dec. 2019), pp.2048-2051.
https://search.emarefa.net/detail/BIM-910228
American Medical Association (AMA)
Yang, Qing& Qiao, Xiaofeng& Li, Daguang& Chen, Bo& Zhang, Lingmin& Yuan, Cuiling…[et al.]. Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 8, pp.2048-2051.
https://search.emarefa.net/detail/BIM-910228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2051
Record ID
BIM-910228