Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center
Joint Authors
Mohajeri, Parviz
Khani, Muhammad Yusuf Ali
Karami, Manoochehr
Karami, Pezhman
Mashouf, Rasul Yusufi
Yaqubi, Mujtaba Hidayat
Dastan, Dara
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1731-1736, 6 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
In burn centers, Pseudomonas aeruginosa acts as a major cause of nosocomial infections.
Therefore, this study aimed to characterize molecularly P.
aeruginosa isolates collected from environmental samples and burn patients.
A total of 78 strains (including 58 clinical and 20 environmental isolates) of the P.
aeruginosa were collected from Beasat hospital of Hamadan, west of Iran, and was identified using API 20NE.
The disk diffusion method according to the CLSI was applied for determination of the antimicrobial resistance.
Moreover, the microtiter plate test was used for the quantification of Biofilm formation.
The genomic features of the isolated strains was evaluated using Pulsed Field Gel Electrophoresis (PFGE).
We found that 94.8% of clinical and 80% environmental isolates were capable of forming biofilm.
The rate of MDR in clinical and environmental isolates was 51.7% and 40%, respectively.
A significant relationship was observed between biofilm formation capability and multiple drug resistance (p < 0.05).
PFGE typing showed 11 different clusters with two major clusters A with 30 (38.5%) and B with 14 (17.9%) members, containing up to 56.4% of all isolates.
There was no relationship between biofilm formation ability and antibiotic resistance patterns with PFGE patterns.
According to the results, the clonal spread of environmental P.
aeruginosa isolates is associated with clinical isolates, and both environmental and clinical isolates are attributed to a high prevalence of the antibiotic resistance and biofilm formation ability.
This study highlighted that the prevention programs should be implemented in the hospital environment to control the spread of P.
aeruginosa in burn units.
American Psychological Association (APA)
Karami, Pezhman& Mohajeri, Parviz& Mashouf, Rasul Yusufi& Karami, Manoochehr& Yaqubi, Mujtaba Hidayat& Dastan, Dara…[et al.]. 2019. Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1731-1736.
https://search.emarefa.net/detail/BIM-910301
Modern Language Association (MLA)
Karami, Pezhman…[et al.]. Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1731-1736.
https://search.emarefa.net/detail/BIM-910301
American Medical Association (AMA)
Karami, Pezhman& Mohajeri, Parviz& Mashouf, Rasul Yusufi& Karami, Manoochehr& Yaqubi, Mujtaba Hidayat& Dastan, Dara…[et al.]. Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1731-1736.
https://search.emarefa.net/detail/BIM-910301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1735-1736
Record ID
BIM-910301