Molecular characterization of toxigenic and antibiotic resistant of staphylococcus aureus of recurrent bovine mastitis
Other Title(s)
الصفات الجزيئية لسموم المكور العنقودي الذهبي المقاوم لبعض المضادات الحيوية لالتهاب الضرع المتكرر فى الأبقار الحلابة
Joint Authors
Qutub, Ibtisam al-Sayyid Zaki
al-Shafii, Muhammad Abd al-Zahir Ahmad
Ibrahim, Samih Abd al-Majid
Source
Assiut Veterinary Medical Journal
Issue
Vol. 64, Issue 158 (31 Jul. 2018), pp.1-8, 8 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2018-07-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To enhance the diagnosis of staphylococcus aureus mastitis and its prospective antibiotic resistance in dairy cattle, a multiplex polymerase chain reaction (pcr) assay was developed for concurrent species identifycation, detection antibiotic resistant phenotypically and genotypically to penicillin (blaz gene), gentamycin (aac(6') aph(2'')genes), and tetracycline (tetk, gene) and recognize the toxogenic patterns of s.
aureus in cow’s milk with recurrent clinical mastitis.
bacterial culturing was carried out on 179 bovine recurrent clinical mastitic milk samples resulted in 84 (46.9%) coagulase positive staph isolates, while 69 (38.5%) confirmed by molecular identification by pcr as s.
aureus.
also some virulence factors as enterotoxin a and b (sea and seb) genes were determined in 7(10.1%) and 1 (1.4%) respectively in confirmed isolates.
as well as determined drugs resistance and their genes.
the results of antimicrobial susceptibility tests showed 100%.
43.5% and 58% resistance for penicillin, gentamycin and tetracycline respectively.
while genotypically were 100%, 40.6% and53.6% for (blaz, aac (6') aph (2'') and tetk, respectively.
this study reports the presence of multidrug resistant s.
aureus in recurrent clinical mastitis with highly virulent toxic genes that could be a major obstacle in the treatment and control of mastitis in dairy farms causing highly economic impacts and recommended that the polymerase chain reaction (pcr)-based assays to detect pathogens associated with mastitis and that it has several advantages, including rapid results and high sensitivity.
American Psychological Association (APA)
Qutub, Ibtisam al-Sayyid Zaki& al-Shafii, Muhammad Abd al-Zahir Ahmad& Ibrahim, Samih Abd al-Majid. 2018. Molecular characterization of toxigenic and antibiotic resistant of staphylococcus aureus of recurrent bovine mastitis. Assiut Veterinary Medical Journal،Vol. 64, no. 158, pp.1-8.
https://search.emarefa.net/detail/BIM-910325
Modern Language Association (MLA)
Qutub, Ibtisam al-Sayyid Zaki…[et al.]. Molecular characterization of toxigenic and antibiotic resistant of staphylococcus aureus of recurrent bovine mastitis. Assiut Veterinary Medical Journal Vol. 64, no. 158 (Jul. 2018), pp.1-8.
https://search.emarefa.net/detail/BIM-910325
American Medical Association (AMA)
Qutub, Ibtisam al-Sayyid Zaki& al-Shafii, Muhammad Abd al-Zahir Ahmad& Ibrahim, Samih Abd al-Majid. Molecular characterization of toxigenic and antibiotic resistant of staphylococcus aureus of recurrent bovine mastitis. Assiut Veterinary Medical Journal. 2018. Vol. 64, no. 158, pp.1-8.
https://search.emarefa.net/detail/BIM-910325
Data Type
Journal Articles
Language
English
Record ID
BIM-910325