Development and validation of Lenalidomide in human plasma by LC-MS-MS
Joint Authors
Ansari, Muhammad Javed
Ranganathan, Premanand
Gunasekaran, V.
Singhvi, Indrajeet
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1843-1847, 5 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
A highly sensitive and ultra-fast high performance liquid chromatography- tandem mass spectrometry (LC–MS/MS) assay is developed and validated for the quantification of Lenalidomide in human plasma.
Lenalidomide is extracted from human plasma by Liquid- Liquid Extraction by Ethyl Acetate and analyzed using a reversed phase isocratic elution on a XTerra RP18, (4.6 × 50 mM, 5 µm) column.
A 0.1% Formic acid: Methanol (10:90% v/v), is used as mobile phase and detection was performed by Triple quadrupole mass spectrometry LC-MS/MS using electrospray ionization in positive mode.
Fluconazole is used as the internal standard.
The lower limit of quantification is 9.999 ng/mL for Lenalidomide.
The calibration curves are consistently accurate and precise over the concentration range of 9.999 to 1010.011 ng/mL in plasma for Lenalidomide.
This novel LC–MS/MS method competes with all the regulatory requirements and shows satisfactory accuracy and precision and is sufficiently sensitive for the performance of pharmacokinetic and bioequivalence studies in humans.
American Psychological Association (APA)
Ranganathan, Premanand& Gunasekaran, V.& Singhvi, Indrajeet& Ansari, Muhammad Javed. 2019. Development and validation of Lenalidomide in human plasma by LC-MS-MS. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1843-1847.
https://search.emarefa.net/detail/BIM-910432
Modern Language Association (MLA)
Ranganathan, Premanand…[et al.]. Development and validation of Lenalidomide in human plasma by LC-MS-MS. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1843-1847.
https://search.emarefa.net/detail/BIM-910432
American Medical Association (AMA)
Ranganathan, Premanand& Gunasekaran, V.& Singhvi, Indrajeet& Ansari, Muhammad Javed. Development and validation of Lenalidomide in human plasma by LC-MS-MS. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1843-1847.
https://search.emarefa.net/detail/BIM-910432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1847
Record ID
BIM-910432