Detection of neospora caninum and coxiella burnetii antibodies in milk and serum of infected dairy cattle

Abstract EN

Coxiella burnetii and Neospora caninum are frequent infectious causes of decreased reproductive outcomes in cattle around the world.

So our study aimed to assess the performance of IgG-ELISA for diagnosis of N.

caninum and C.

burnetii and compare the sensitivity of ELISA and IFA in detection IgG to C.burnetii phase II,to achieve these, a total of 200 samples including milk and serum samples (100 for each) were collected from100 dairy cattle suffering from infertility and abortion during the period from January to December 2017 in four governorates of upper Egypt, these samples were transferred to laboratory for serological examination.

The results revealed that N.caninum antibodies were detected in 29% of serum samples and 10% of milk samples while C.burnetii antibodies were detected in 34% and 17% of serum and milk samples respectively.

The IgGELISA of both N.caninum and C.burnetii were detected in the same serum and milk samples with percentage14% and 4% respectively, amid dairy cattle in 4 governorates of upper Egypt.

IgM-IFA indicated the acute infection of C.burnetii in 11% and 4% of serum and milk samples while chronic infection (IgG-IFA) was detected in 19% of serum samples and 8% of milk samples.

The comparison between sensitivity of ELISA and IFA in detection of IGg for C.burnetii phase II showed that ELISA was more sensitive and excellent diagnostic method for C.burnetii infection.