Serodetection and molecular confirmation of mycobacterium tuberculosis infections in dogs

Abstract EN

The aim of the present study was to detect the prevalence rate of IgG-antibodies specific for M.

tuberculosis in dogs by application of indirect ELISA and confirming the infection in seropositive samples by using a molecular test (PCR).

For this purpose, 178 stray dogs were selected randomly from some regions of Al-Qadisiyyah governorate / Iraq, for blood samples collection.

The total prevalence rate of seropositive was 11 (6.18 %) that subjected for culture, and the suspected samples were tested by PCR technique that revealed on 6 (3.37 %) positive samples.

Also, the relationships, between the positive infections with sex and age factors, had been studied.

The study revealed that the infection ratio in males and females was 5.41 % and 6.38 % serologically, and 2.7 % and 3.55 % molecularly, respectively.

In concerning to age factor, the morbidity rate of tuberculosis in ? 1 year and ? 1 year by ELISA was 1.09 % and 11.63 %, whilst it was 0 % and 6.98 % with PCR, respectively.

Statistically, at P? 0.05 level, the significant differences were reported between positive samples by both (indirect ELISA and PCR) tests, and between age groups, whist, it was not showed between sex groups.