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Effect of extracellular vesicles derived from mesenchymal stem cells on k-562 leukemia cell line
Joint Authors
Valandani, Hajir Mardani
Amirizadeh, Nasir
Nikougoftar, Mahin
Safa, Majid
Khalilabadi, Roohollah Mirzaee
al-Kazimi, Ahmad
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 20, Issue 5 (31 May. 2018), pp.1-8, 8 p.
Publisher
Publication Date
2018-05-31
Country of Publication
United Arab Emirates
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background: Several studies show that mesenchymal stem cells (MSCs) possess anti-tumor properties, while other studies show that MSCs may promote cancer.
Extracellular vesicles (EVs) are identified as novel mediators to communicate among cells, which may be used as vehicles to transfer the MSC information to the target cells.
Objectives: The current study aimed at investigating whether the human umbilical cord mesenchymal stem cell (HUSCMSC) derived extracellular vesicles (EVs) inhibits or promotes tumor cell growth in K562 chronic myelogenous leukemia cell line.
Methods: In the current experimental study, trypan blue exclusion and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay were performed to investigate the cell viability and metabolic activity.
Additionally, flow cytometry was employed to assess cell cycle and apoptosis of K562 cells after 72 hours exposure to EVs.
Results: The obtained results showed that no doses of EVs inhibited the tumor growth (mean standard deviation (SD) of cell viability at the day 10 was 94.42.60, P > 0.05) and metabolic activity in three days (meanSD at the day 3 was 97.272.46, P > 0.05).
Furthermore, EVs also did not change the apoptosis rate (meanSD at the day 3 was 3.172.34 for annexin positive cells, and 3.361.91 for annexin/propidium iodide (PI) -positive cells, P > 0.05).
Conclusions: In conclusion, no significant changes in tumor cell growth were observed after treating K562 cells with MSCs-derived EVs.
American Psychological Association (APA)
Valandani, Hajir Mardani& Amirizadeh, Nasir& Nikougoftar, Mahin& Safa, Majid& Khalilabadi, Roohollah Mirzaee& al-Kazimi, Ahmad. 2018. Effect of extracellular vesicles derived from mesenchymal stem cells on k-562 leukemia cell line. Iranian Red Crescent Medical Journal،Vol. 20, no. 5, pp.1-8.
https://search.emarefa.net/detail/BIM-912235
Modern Language Association (MLA)
Valandani, Hajir Mardani…[et al.]. Effect of extracellular vesicles derived from mesenchymal stem cells on k-562 leukemia cell line. Iranian Red Crescent Medical Journal Vol. 20, no. 5 (May. 2018), pp.1-8.
https://search.emarefa.net/detail/BIM-912235
American Medical Association (AMA)
Valandani, Hajir Mardani& Amirizadeh, Nasir& Nikougoftar, Mahin& Safa, Majid& Khalilabadi, Roohollah Mirzaee& al-Kazimi, Ahmad. Effect of extracellular vesicles derived from mesenchymal stem cells on k-562 leukemia cell line. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 5, pp.1-8.
https://search.emarefa.net/detail/BIM-912235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p.6-8
Record ID
BIM-912235