Detection of B-lactamases producing methicillin-resistant staphylococcus aureus (MRSA)‎

Abstract EN

Methicillin resistant staphylococcus aureus (MRSA) is one of the most important causes of nosocomial and community infections and most clinical isolates are β-lactams and multidrug resistant.

resistance to β-lactam antibiotics is most often due to penicillin binding proteins (PBPS) and β-lactamases production.

characterization of β-lactamases is important for choosing appropriate antibiotic therapy, therefore 135 s.

aureus were collected from 343 clinical samples between august 2012 and January 2013 from several clinical sources that were randomly selected from patients in three main hospitals in al-Najaf city and the prevalence of MRSA in Najaf hospitals was 64 (47.4%) and the remaining 71 (52.6%) isolates were MSSA, and all MRSA isolates were tested for β-lactamase production by using seven methods: [cloverleaf test, Masuda double-disc test, iodometric method, acidometric method, chromogenic (nitrocefine) method, double disc method and penicillin disc diffusion method].

the findings of this study revealed that chromogenic (nitrocefine) method, cloverleaf test and Masuda test had a high accuracy (100%) comparative with other methods tested for detection of MRSA β-lactamase production.